Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SRY CRISPR/Cas9 KO Plasmid (h): sc-404342
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SRY CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SRY genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SRY Antibody (SRY-15): sc-69842
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRY CRISPR/Cas9 KO Plasmid (h) | sc-404342 | 20 µg | $397.00 |
Overview
SRY encodes the sex-determining region Y transcription factor, an HMG-box DNA-binding protein that initiates male sex determination by directing early gonadal differentiation toward testis development. By regulating transcriptional programs in the bipotential gonad, SRY drives downstream pathways including activation of SOX9 and broader gene networks that coordinate Sertoli cell lineage commitment and suppression of ovarian-promoting signals. Disruption or dysregulation of SRY function is associated with disorders of sex development, including 46,XY gonadal dysgenesis and sex reversal phenotypes, making it a key target for studying transcriptional control of developmental fate decisions. In cellular models, SRY serves as a tractable node for interrogating chromatin-dependent gene regulation and lineage-specifying transcription factor activity.
SRY CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SRY gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SRY together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SRY open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SRY protein expression.
This CRISPR knockout system enables efficient generation of SRY-deficient cell models for investigation of SRY signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SRY exon(s) critical for SRY function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SRY genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SRY CRISPR/Cas9 KO Plasmid (h) and SRY CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SRY locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SRY HDR Plasmid (h) and SRY HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SRY homology arms to support homology-directed repair at defined SRY target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.