Shigatoxin 2 Antibody (11E10): sc-52727

Shigatoxin 2 Antibody (11E10) is a mouse monoclonal IgG1 antibody that detects Shigatoxin 2 protein of E. coli origin by western blotting (WB). Anti-Shigatoxin 2 antibody (11E10) is available as a non-conjugated format, providing researchers with a reliable tool for studying this critical protein. Shigatoxin 2 (Stx2) is a potent virulence factor secreted by enterohemorrhagic Escherichia coli, particularly Shigella dysenteriae type 1, and plays a significant role in the pathogenesis of hemolytic uremic syndrome (HUS), which is the leading cause of acute renal failure in children globally. Stx2′s primary function involves inhibiting protein synthesis in host cells, leading to cell death and contributing to the inflammatory response that characterizes HUS. This mechanism disrupts normal cellular functions and triggers a cascade of immune responses that exacerbate tissue damage, particularly in the kidneys. Understanding Stx2′s role in disease progression is vital for developing therapeutic strategies aimed at mitigating the severe effects of infections caused by Shigella and E. coli.

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Shigatoxin 2 Antibody (11E10) References:

1. Occurrence and characterization of Escherichia coli O157 isolated from cattle in Norway.  |  Vold, L., et al. 2001. Vet Res Commun. 25: 13-26. PMID: 11214668
2. Antibody response to Shiga toxins Stx2 and Stx1 in children with enteropathic hemolytic-uremic syndrome.  |  Ludwig, K., et al. 2001. J Clin Microbiol. 39: 2272-9. PMID: 11376069
3. Shiga toxin-producing Escherichia coli infection and antibodies against Stx2 and Stx1 in household contacts of children with enteropathic hemolytic-uremic syndrome.  |  Ludwig, K., et al. 2002. J Clin Microbiol. 40: 1773-82. PMID: 11980959
4. Cytokine expression in the renal tubular epithelial cells stimulated by Shiga toxin 2 of Escherichia coli O157:H7.  |  Lee, JE., et al. 2002. Ren Fail. 24: 567-75. PMID: 12380901
5. Cytotoxic effect of Shiga toxin-2 holotoxin and its B subunit on human renal tubular epithelial cells.  |  Creydt, VP., et al. 2006. Microbes Infect. 8: 410-9. PMID: 16242986
6. Shiga toxin 2 and lipopolysaccharide induce human microvascular endothelial cells to release chemokines and factors that stimulate platelet function.  |  Guessous, F., et al. 2005. Infect Immun. 73: 8306-16. PMID: 16299328
7. Shiga toxin 2 and lipopolysaccharide cause monocytic THP-1 cells to release factors which activate platelet function.  |  Guessous, F., et al. 2005. Thromb Haemost. 94: 1019-27. PMID: 16366002
8. Human serum amyloid P component protects against Escherichia coli O157:H7 Shiga toxin 2 in vivo: therapeutic implications for hemolytic-uremic syndrome.  |  Armstrong, GD., et al. 2006. J Infect Dis. 193: 1120-4. PMID: 16544252
9. Direct acute tubular damage contributes to Shigatoxin-mediated kidney failure.  |  Porubsky, S., et al. 2014. J Pathol. 234: 120-33. PMID: 24909663
10. Platelet-delivered ADAMTS13 inhibits arterial thrombosis and prevents thrombotic thrombocytopenic purpura in murine models.  |  Pickens, B., et al. 2015. Blood. 125: 3326-34. PMID: 25800050
11. ADAMTS13 or Caplacizumab Reduces the Accumulation of Neutrophil Extracellular Traps and Thrombus in Whole Blood of COVID-19 Patients under Flow.  |  Yada, N., et al. 2024. Thromb Haemost. 124: 725-738. PMID: 38272066
12. Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay.  |  Perera, LP., et al. 1988. J Clin Microbiol. 26: 2127-31. PMID: 3053764

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Shigatoxin 2 Antibody (11E10) has 5 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Shigatoxin 2 Antibody (11E10) Specifications

Alternate Product Name: Shigatoxin 2

Product Clone Name: 11E10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Shigatoxin 2

Epitope Species: E. coli

Product Reactivity/Detection: Shigatoxin 2

Species Reactivity/Detection: E. coli

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Shigatoxin 2 Antibody (11E10): N/A