Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SAPK4 CRISPR/Cas9 KO Plasmid (h): sc-400423
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SAPK4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SAPK4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SAPK4 Antibody (E-7): sc-46678
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAPK4 CRISPR/Cas9 KO Plasmid (h) | sc-400423 | 20 µg | $397.00 |
Overview
MAPK13 encodes stress-activated protein kinase 4 (SAPK4), a p38 MAPK family serine/threonine kinase that transduces inflammatory and environmental stress signals into transcriptional and post-transcriptional responses. SAPK4 contributes to regulation of cytokine-associated signaling, cellular differentiation programs, and context-dependent control of apoptosis and proliferation through phosphorylation of downstream effectors. As part of the broader MAPK network, MAPK13/SAPK4 interfaces with pathways that shape innate immune responses and epithelial biology. Dysregulated p38 MAPK signaling, including MAPK13 activity, has been linked to inflammatory pathophysiology and cancer-associated processes, making it a useful node for mechanistic studies of stress signaling.
SAPK4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MAPK13 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MAPK13 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MAPK13 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SAPK4 protein expression.
This CRISPR knockout system enables efficient generation of MAPK13-deficient cell models for investigation of SAPK4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MAPK13 exon(s) critical for SAPK4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MAPK13 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SAPK4 CRISPR/Cas9 KO Plasmid (h) and SAPK4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MAPK13 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SAPK4 HDR Plasmid (h) and SAPK4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MAPK13 homology arms to support homology-directed repair at defined MAPK13 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.