Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Sam 68 CRISPR/Cas9 KO Plasmid (m): sc-422795
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sam 68 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sam 68 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sam 68 Antibody (H-4): sc-514468
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sam 68 CRISPR/Cas9 KO Plasmid (m) | sc-422795 | 20 µg | $397.00 |
Overview
Khdrbs1 encodes the RNA-binding protein Sam 68 (KHDRBS1), a STAR family regulator of post-transcriptional gene expression that couples signal transduction to RNA metabolism. Sam 68 binds specific RNA elements to control alternative splicing, mRNA export, stability, and translation, and it participates in dynamic ribonucleoprotein complexes including stress granule–associated pathways. Through phosphorylation-dependent interactions with Src-family kinases and other signaling nodes, Sam 68 influences cell-cycle progression, apoptosis, and differentiation programs. Dysregulation of KHDRBS1/Sam 68–mediated splicing and RNA processing has been linked to oncogenic signaling, neurodevelopmental phenotypes, and immune-related transcriptional states, supporting its broad utility in mechanistic studies.
Sam 68 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Khdrbs1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Khdrbs1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Khdrbs1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sam 68 protein expression.
This CRISPR knockout system enables efficient generation of Khdrbs1-deficient cell models for investigation of Sam 68 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Khdrbs1 exon(s) critical for Sam 68 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Khdrbs1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sam 68 CRISPR/Cas9 KO Plasmid (m) and Sam 68 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Khdrbs1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sam 68 HDR Plasmid (m) and Sam 68 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Khdrbs1 homology arms to support homology-directed repair at defined Khdrbs1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.