RNF8 Antibody (B-2): sc-271462
- RNF8 Antibody (B-2) is a mouse monoclonal IgG2a κ RNF8 antibody, cited in 58 publications, provided at 200 µg/ml
- raised against amino acids 186-485 mapping at the C-terminus of RNF8 of human origin
- RNF8 Antibody (B-2) is recommended for detection of RNF8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-RNF8 Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RNF8 Antibody (B-2) for WB and IHC(P) applications. These reagents are now offered in bundles with RNF8 Antibody (B-2) (see ordering information below).
RNF8 Antibody (B-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects RNF8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-RNF8 antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNF8, also known as Ring Finger Protein 8, plays a crucial role in the ubiquitin-proteasome pathway, functioning as an E3 ubiquitin ligase that facilitates the ubiquitination of target proteins, which is essential for their degradation and regulation within the cell. This process is vital for maintaining cellular homeostasis and regulating various cellular functions, including DNA repair, cell cycle progression, and apoptosis. RNF8 is predominantly located in the nucleus, where RNF8 interacts with the heterodimeric ubiquitin-conjugating enzyme UBC13, co-localizing to promote polyubiquitination of substrates. Additionally, RNF8 has been shown to bind to Retinoid X receptor alpha (RXRα), enhancing RXRα transcriptional activity, which underscores RNF8′s importance in gene regulation. RNF8′s ability to modulate RXRα-mediated transcription further highlights RNF8′s role in cellular signaling pathways, making RNF8 a significant target for research in cancer and other diseases where ubiquitination processes are disrupted.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RNF8 Antibody (B-2) References:
- The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. | Moynihan, TP., et al. 1999. J Biol Chem. 274: 30963-8. PMID: 10521492
- Interaction of the ring finger-related U-box motif of a nuclear dot protein with ubiquitin-conjugating enzymes. | Pringa, E., et al. 2001. J Biol Chem. 276: 19617-23. PMID: 11274149
- N-Terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8. | Ito, K., et al. 2001. Eur J Biochem. 268: 2725-32. PMID: 11322894
- Protein-protein interactions within an E2-RING finger complex. Implications for ubiquitin-dependent DNA damage repair. | Ulrich, HD. 2003. J Biol Chem. 278: 7051-8. PMID: 12496280
- Small nuclear RING finger protein expression during gonad development: regulation by gonadotropins and estrogen in the postnatal ovary. | Hirvonen-Santti, SJ., et al. 2004. Endocrinology. 145: 2433-44. PMID: 14749358
- The RING finger protein, RNF8, interacts with retinoid X receptor alpha and enhances its transcription-stimulating activity. | Takano, Y., et al. 2004. J Biol Chem. 279: 18926-34. PMID: 14981089
- The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation. | Plans, V., et al. 2006. J Cell Biochem. 97: 572-82. PMID: 16215985
- The RING-finger protein haprin: domains and function in the acrosome reaction. | Kitamura, K., et al. 2005. Curr Protein Pept Sci. 6: 567-74. PMID: 16381605
- How a RING finger protein and a steroid hormone control autophagy. | Lindmo, K. and Stenmark, H. 2006. Autophagy. 2: 321-2. PMID: 16874068
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF8 Antibody (B-2) | sc-271462 | 200 µg/ml | $322.00 | |||
RNF8 Antibody (B-2): m-IgG Fc BP-HRP Bundle | sc-529189 | 200 µg Ab; 10 µg BP | $361.00 | |||
RNF8 Antibody (B-2): m-IgGκ BP-HRP Bundle | sc-521950 | 200 µg Ab, 40 µg BP | $361.00 | |||
RNF8 Antibody (B-2): m-IgG2a BP-HRP Bundle | sc-550934 | 200 µg Ab; 10 µg BP | $361.00 | |||
RNF8 Antibody (B-2) AC | sc-271462 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RNF8 Antibody (B-2) HRP | sc-271462 HRP | 200 µg/ml | $322.00 | |||
RNF8 Antibody (B-2) FITC | sc-271462 FITC | 200 µg/ml | $336.00 | |||
RNF8 Antibody (B-2) PE | sc-271462 PE | 200 µg/ml | $349.00 | |||
RNF8 Antibody (B-2) Alexa Fluor® 488 | sc-271462 AF488 | 200 µg/ml | $364.00 | |||
RNF8 Antibody (B-2) Alexa Fluor® 546 | sc-271462 AF546 | 200 µg/ml | $364.00 | |||
RNF8 Antibody (B-2) Alexa Fluor® 594 | sc-271462 AF594 | 200 µg/ml | $364.00 | |||
RNF8 Antibody (B-2) Alexa Fluor® 647 | sc-271462 AF647 | 200 µg/ml | $364.00 | |||
RNF8 Antibody (B-2) Alexa Fluor® 680 | sc-271462 AF680 | 200 µg/ml | $364.00 | |||
RNF8 Antibody (B-2) Alexa Fluor® 790 | sc-271462 AF790 | 200 µg/ml | $364.00 |