PLC β3 Antibody (D-7): sc-133231
- PLC β3 Antibody (D-7) is a mouse monoclonal IgG2a κ PLC β3 antibody, cited in 13 publications, provided at 200 µg/ml
- raised against amino acids 1151-1234 of PLC β3 of human origin
- PLC beta 3 Antibody (D-7) is recommended for detection of PLC β3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-PLC beta 3 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PLC β3 Antibody (D-7) for WB and IHC(P) applications. These reagents are now offered in bundles with PLC β3 Antibody (D-7) (see ordering information below).
PLC β3 Antibody (D-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects PLC beta 3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PLC β3 (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PLC beta 3 protein plays a vital role in cellular signaling pathways by catalyzing the hydrolysis of phosphatidylinositol 4, 5-bisphosphate into inositol 1, 4, 5-trisphosphate and diacylglycerol, which are crucial second messengers in regulating various physiological processes, including cell growth, differentiation, and apoptosis. PLC beta 3 is predominantly located in the plasma membrane, where PLC beta 3 interacts with G protein-coupled receptors and is activated by Gq class heterotrimeric G proteins, highlighting its importance in mediating receptor signaling. PLC beta 3 presence in various tissues underscores its significance in both normal physiology and pathological conditions, making PLC beta 3 a valuable target for research and therapeutic applications.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PLC β3 Antibody (D-7) References:
- Regulation of inositol phospholipid-specific phospholipase C isozymes. | Rhee, SG. and Choi, KD. 1992. J Biol Chem. 267: 12393-6. PMID: 1319994
- SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins. | Koch, CA., et al. 1991. Science. 252: 668-74. PMID: 1708916
- A second gene product of the inositol-phospholipid-specific phospholipase C delta subclass. | Meldrum, E., et al. 1991. Eur J Biochem. 196: 159-65. PMID: 1848183
- Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5. | Xiao, W., et al. 2009. Cancer Cell. 16: 161-71. PMID: 19647226
- Full-length Gα(q)-phospholipase C-β3 structure reveals interfaces of the C-terminal coiled-coil domain. | Lyon, AM., et al. 2013. Nat Struct Mol Biol. 20: 355-62. PMID: 23377541
- M3 muscarinic receptor interaction with phospholipase C β3 determines its signaling efficiency. | Kan, W., et al. 2014. J Biol Chem. 289: 11206-11218. PMID: 24596086
- A second type of rat phosphoinositide-specific phospholipase C containing a src-related sequence not essential for phosphoinositide-hydrolyzing activity. | Emori, Y., et al. 1989. J Biol Chem. 264: 21885-90. PMID: 2557343
- Inositol phospholipid-specific phospholipase C: complete cDNA and protein sequences and sequence homology to tyrosine kinase-related oncogene products. | Suh, PG., et al. 1988. Proc Natl Acad Sci U S A. 85: 5419-23. PMID: 2840660
- Cloning of cDNA encoding rat phospholipase C-beta 4, a new member of the phospholipase C. | Kim, MJ., et al. 1993. Biochem Biophys Res Commun. 194: 706-12. PMID: 7688223
- Activation of phospholipase C beta 2 by the alpha and beta gamma subunits of trimeric GTP-binding protein. | Wu, D., et al. 1993. Proc Natl Acad Sci U S A. 90: 5297-301. PMID: 8389480
- Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3. | Jhon, DY., et al. 1993. J Biol Chem. 268: 6654-61. PMID: 8454637
- Contribution of phospholipase C-beta3 phosphorylation to the rapid attenuation of opioid-activated phosphoinositide response. | Strassheim, D., et al. 1998. Mol Pharmacol. 53: 1047-53. PMID: 9614207
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLC β3 Antibody (D-7) | sc-133231 | 200 µg/ml | $322.00 | |||
PLC β3 Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-528777 | 200 µg Ab; 10 µg BP | $361.00 | |||
PLC β3 Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-521284 | 200 µg Ab, 40 µg BP | $361.00 | |||
PLC β3 Antibody (D-7): m-IgG2a BP-HRP Bundle | sc-547169 | 200 µg Ab; 10 µg BP | $361.00 | |||
PLC β3 Antibody (D-7) AC | sc-133231 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PLC β3 Antibody (D-7) HRP | sc-133231 HRP | 200 µg/ml | $322.00 | |||
PLC β3 Antibody (D-7) FITC | sc-133231 FITC | 200 µg/ml | $336.00 | |||
PLC β3 Antibody (D-7) PE | sc-133231 PE | 200 µg/ml | $349.00 | |||
PLC β3 Antibody (D-7) Alexa Fluor® 488 | sc-133231 AF488 | 200 µg/ml | $364.00 | |||
PLC β3 Antibody (D-7) Alexa Fluor® 546 | sc-133231 AF546 | 200 µg/ml | $364.00 | |||
PLC β3 Antibody (D-7) Alexa Fluor® 594 | sc-133231 AF594 | 200 µg/ml | $364.00 | |||
PLC β3 Antibody (D-7) Alexa Fluor® 647 | sc-133231 AF647 | 200 µg/ml | $364.00 | |||
PLC β3 Antibody (D-7) Alexa Fluor® 680 | sc-133231 AF680 | 200 µg/ml | $364.00 | |||
PLC β3 Antibody (D-7) Alexa Fluor® 790 | sc-133231 AF790 | 200 µg/ml | $364.00 |