p-GSK3 beta Antibody (2D3): sc-81494

p-GSK-3β Antibody (2D3) is a mouse monoclonal IgG1 antibody that detects glycogen synthase kinase-3β (GSK-3β) in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. Anti-p-GSK-3β antibody (2D3) specifically recognizes the phosphorylated form of GSK-3β, which plays a crucial role in various cellular processes, including energy metabolism, cell proliferation, and apoptosis regulation. GSK-3β is primarily located in the cytoplasm but can translocate to the nucleus, where GSK-3β influences the activity of transcription factors and other signaling pathways. This subcellular localization is vital because GSK-3β modulates gene expression in response to extracellular signals, thereby impacting cellular responses to growth factors and stress. The phosphorylation state of GSK-3β, particularly at Ser 9, is a key regulatory mechanism that determines activity; when phosphorylated, GSK-3β is inactivated, which is essential for promoting cell survival and growth. GSK-3β interacts with various proteins, including cAMP-dependent protein kinase A, which also phosphorylates GSK-3β, highlighting the intricate regulatory networks that control function. p-GSK-3β (2D3) antibody specifically detects phosphorylated GSK-3β, making p-GSK-3β (2D3) antibody an invaluable tool for researchers studying the signaling pathways involved in cell growth and survival.

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p-GSK3 beta Antibody (2D3) References:

1. The structure of phosphorylated GSK-3beta complexed with a peptide, FRATtide, that inhibits beta-catenin phosphorylation.  |  Bax, B., et al. 2001. Structure. 9: 1143-52. PMID: 11738041
2. Glycogen synthase kinase-3beta inhibits the xenobiotic and antioxidant cell response by direct phosphorylation and nuclear exclusion of the transcription factor Nrf2.  |  Salazar, M., et al. 2006. J Biol Chem. 281: 14841-51. PMID: 16551619
3. Lithium increases platelet serine-9 phosphorylated GSK-3β levels in drug-free bipolar disorder during depressive episodes.  |  de Sousa, RT., et al. 2015. J Psychiatr Res. 62: 78-83. PMID: 25691093
4. Transient Cerebral Ischemia Alters GSK-3β and p-GSK-3β Immunoreactivity in Pyramidal Neurons and Induces p-GSK-3β Expression in Astrocytes in the Gerbil Hippocampal CA1 Area.  |  Chen, BH., et al. 2017. Neurochem Res. 42: 2305-2313. PMID: 28349361
5. Stilbenoid pterostilbene complexed with cyclodextrin preserves left ventricular function after myocardial infarction in rats: possible involvement of thiol proteins and modulation of phosphorylated GSK-3β.  |  Lacerda, D., et al. 2018. Free Radic Res. 52: 988-999. PMID: 30203709
6. Curcumin ameliorates scopolamine-induced mice memory retrieval deficit and restores hippocampal p-Akt and p-GSK-3β.  |  SoukhakLari, R., et al. 2018. Eur J Pharmacol. 841: 28-32. PMID: 30321530
7. Inflammation has synergistic effect with nicotine in periodontitis by up-regulating the expression of α7 nAChR via phosphorylated GSK-3β.  |  Zhou, Z., et al. 2020. J Cell Mol Med. 24: 2663-2676. PMID: 31930698
8. Phosphorylated GSK‑3β protects stress‑induced apoptosis of myoblasts via the PI3K/Akt signaling pathway.  |  Liu, M., et al. 2020. Mol Med Rep. 22: 317-327. PMID: 32377749
9. Increasing Odontoblast-like Differentiation from Dental Pulp Stem Cells through Increase of β-Catenin/p-GSK-3β Expression by Low-Frequency Electromagnetic Field.  |  Lim, HM., et al. 2021. Biomedicines. 9: PMID: 34440255
10. Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.  |  He, Z., et al. 2021. J Oncol. 2021: 4475192. PMID: 34966427

The preferred GSK3 alpha/beta antibody is GSK3 alpha/beta Antibody (0011-A): sc-7291.