ODC Double Nickase Plasmid (m): sc-422006-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ODC Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ODC Double Nickase Plasmid (m) and ODC Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Odc1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ODC Antibody (E-6): sc-398116
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ODC Double Nickase Plasmid (m) | sc-422006-NIC | 20 µg | $410.00 | |||
ODC Double Nickase Plasmid (m2) | sc-422006-NIC-2 | 20 µg | $410.00 |
Mouse Odc1 encodes ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis that converts ornithine to putrescine, supporting spermidine and spermine production. ODC activity integrates metabolic and growth signaling to regulate proliferation, translation, and chromatin dynamics, with tight control through antizyme-mediated feedback and proteasomal turnover. Perturbation of Odc1 impacts polyamine-dependent processes including cell-cycle progression, stress responses, and differentiation, making it relevant to studies of dysregulated growth programs and metabolic remodeling.
ODC Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Odc1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Odc1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Odc1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Odc1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.