Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NF-1X CRISPR/Cas9 KO Plasmid (m): sc-421875
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NF-1X CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NF-1X genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NF-1 Antibody (D-2): sc-74444
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NF-1X CRISPR/Cas9 KO Plasmid (m) | sc-421875 | 20 µg | $397.00 |
Overview
Nfix encodes the nuclear factor I X-type (NF-1X), a sequence-specific DNA-binding transcription factor that recognizes NFI consensus elements to regulate promoter and enhancer activity during development. In mouse, NF-1X contributes to lineage specification and maturation programs in the nervous system and skeletal muscle, influencing cell-cycle exit, differentiation, and extracellular matrix–associated gene expression. NF-1 family factors interface with chromatin remodeling and transcriptional co-regulator networks to coordinate developmental timing and tissue architecture. Dysregulated NFIX function has been linked to developmental syndromes and altered growth and differentiation phenotypes, making Nfix a useful node for studying gene regulatory circuits relevant to neurodevelopment and myogenesis.
NF-1X CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nfix gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nfix together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nfix open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NF-1X protein expression.
This CRISPR knockout system enables efficient generation of Nfix-deficient cell models for investigation of NF-1X signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nfix exon(s) critical for NF-1X function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nfix genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NF-1X CRISPR/Cas9 KO Plasmid (m) and NF-1X CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nfix locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NF-1X HDR Plasmid (m) and NF-1X HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nfix homology arms to support homology-directed repair at defined Nfix target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.