myocilin Antibody (F-12): sc-137233
- myocilin Antibody (F-12) is a mouse monoclonal IgG2b κ myocilin antibody, cited in 12 publications, provided at 200 µg/ml
- raised against amino acids 240-370 mapping within an internal region of myocilin of human origin
- myocilin Antibody (F-12) is recommended for detection of myocilin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-myocilin Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for myocilin Antibody (F-12) for WB applications. These reagents are now offered in bundles with myocilin Antibody (F-12) (see ordering information below).
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myocilin Antibody (F-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects myocilin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-myocilin antibody (F-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myocilin is an extracellular protein predominantly expressed in the eye, particularly in the retina, trabecular meshwork, and ciliary body, where myocilin plays a crucial role in maintaining intraocular pressure (IOP). Myocilin′s ability to form homomultimers in vivo and interaction with components of the extracellular matrix, such as the Hep II domain of fibronectin, highlight myocilin′s importance in structural integrity and cellular signaling within the ocular environment. Additionally, myocilin interacts with myosin regulatory light chain, a key component of the myosin motor protein complex, suggesting myocilin′s involvement in the actomyosin system, which is essential for the proper functioning of the trabecular meshwork. Disruptions in this system can lead to elevated IOP and contribute to the development of glaucoma, a leading cause of blindness worldwide. Myocilin is encoded by the MYOC gene, also known as TIGR, located at the GLC1A locus on chromosome 1q24.3, and mutations in myocilin are specifically associated with primary open-angle glaucoma (POAG), a progressive condition characterized by the loss of retinal ganglion cells.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
myocilin Antibody (F-12) References:
- Targeted Disruption of the Myocilin Gene (Myoc) Suggests that Human Glaucoma-Causing Mutations Are Gain of Function. | Kim, BS., et al. 2001. Mol Cell Biol. 21: 7707-13. PMID: 11604506
- In vitro localization of TIGR/MYOC in trabecular meshwork extracellular matrix and binding to fibronectin. | Filla, MS., et al. 2002. Invest Ophthalmol Vis Sci. 43: 151-61. PMID: 11773026
- Protein interactions with myocilin. | Wentz-Hunter, K., et al. 2002. Invest Ophthalmol Vis Sci. 43: 176-82. PMID: 11773029
- Myocilin is associated with mitochondria in human trabecular meshwork cells. | Wentz-Hunter, K., et al. 2002. J Cell Physiol. 190: 46-53. PMID: 11807810
- Expression of myocilin/TIGR in normal and glaucomatous primate optic nerves. | Ricard, CS., et al. 2001. Exp Eye Res. 73: 433-47. PMID: 11825016
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
myocilin Antibody (F-12) | sc-137233 | 200 µg/ml | $322.00 | |||
myocilin Antibody (F-12): m-IgGκ BP-HRP Bundle | sc-521462 | 200 µg Ab, 40 µg BP | $361.00 | |||
myocilin Antibody (F-12): m-IgG2b BP-HRP Bundle | sc-548906 | 200 µg Ab; 10 µg BP | $361.00 | |||
myocilin Antibody (F-12) AC | sc-137233 AC | 500 µg/ml, 25% agarose | $424.00 | |||
myocilin Antibody (F-12) HRP | sc-137233 HRP | 200 µg/ml | $322.00 | |||
myocilin Antibody (F-12) FITC | sc-137233 FITC | 200 µg/ml | $336.00 | |||
myocilin Antibody (F-12) PE | sc-137233 PE | 200 µg/ml | $349.00 | |||
myocilin Antibody (F-12) Alexa Fluor® 488 | sc-137233 AF488 | 200 µg/ml | $364.00 | |||
myocilin Antibody (F-12) Alexa Fluor® 546 | sc-137233 AF546 | 200 µg/ml | $364.00 | |||
myocilin Antibody (F-12) Alexa Fluor® 594 | sc-137233 AF594 | 200 µg/ml | $364.00 | |||
myocilin Antibody (F-12) Alexa Fluor® 647 | sc-137233 AF647 | 200 µg/ml | $364.00 | |||
myocilin Antibody (F-12) Alexa Fluor® 680 | sc-137233 AF680 | 200 µg/ml | $364.00 | |||
myocilin Antibody (F-12) Alexa Fluor® 790 | sc-137233 AF790 | 200 µg/ml | $364.00 |