LIS1 Antibody (H-7): sc-374586

LIS1 Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects LIS1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). LIS1 (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LIS1 protein plays a critical role in neuronal migration during brain development, and LIS1 dysfunction is associated with lissencephaly, a condition characterized by a smooth cerebral surface and severe cognitive impairments. LIS1 protein is essential for proper organization of the cerebral cortex, as LIS1 interacts with doublecortin, another protein implicated in neuronal migration. LIS1 interaction with doublecortin is vital for regulating microtubule dynamics, which are crucial for neuron movement to appropriate locations in the developing brain. Disruptions in this process can lead to significant developmental disorders, highlighting the importance of studying LIS1 and associated pathways.

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LIS1 Antibody (H-7) References:

1. LIS1 and dynein motor function in neuronal migration and development.  |  Wynshaw-Boris, A. and Gambello, MJ. 2001. Genes Dev. 15: 639-51. PMID: 11274050
2. Ndel1 operates in a common pathway with LIS1 and cytoplasmic dynein to regulate cortical neuronal positioning.  |  Shu, T., et al. 2004. Neuron. 44: 263-77. PMID: 15473966
3. Binding of microtubule-associated protein 1B to LIS1 affects the interaction between dynein and LIS1.  |  Jiménez-Mateos, EM., et al. 2005. Biochem J. 389: 333-41. PMID: 15762842
4. Dictyostelium LIS1 is a centrosomal protein required for microtubule/cell cortex interactions, nucleus/centrosome linkage, and actin dynamics.  |  Rehberg, M., et al. 2005. Mol Biol Cell. 16: 2759-71. PMID: 15800059
5. LIS1 controls mitosis and mitotic spindle organization via the LIS1-NDEL1-dynein complex.  |  Moon, HM., et al. 2014. Hum Mol Genet. 23: 449-66. PMID: 24030547
6. Microtubule-binding-induced allostery triggers LIS1 dissociation from dynein prior to cargo transport.  |  Ton, WD., et al. 2023. Nat Struct Mol Biol. 30: 1365-1379. PMID: 37322240
7. Isolation of a Miller-Dieker lissencephaly gene containing G protein beta-subunit-like repeats.  |  Reiner, O., et al. 1993. Nature. 364: 717-21. PMID: 8355785
8. Folding of proteins with WD-repeats: comparison of six members of the WD-repeat superfamily to the G protein beta subunit.  |  Garcia-Higuera, I., et al. 1996. Biochemistry. 35: 13985-94. PMID: 8909296
9. Platelet-activating factor acetylhydrolase expression and activity suggest a link between neuronal migration and platelet-activating factor.  |  Albrecht, U., et al. 1996. Dev Biol. 180: 579-93. PMID: 8954729
10. A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome.  |  des Portes, V., et al. 1998. Cell. 92: 51-61. PMID: 9489699
11. Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein.  |  Gleeson, JG., et al. 1998. Cell. 92: 63-72. PMID: 9489700
12. LISsen up!  |  Walsh, CA. 1998. Nat Genet. 19: 307-8. PMID: 9697681

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LIS1 Antibody (H-7) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-LIS1 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LIS1 Antibody (H-7) Specifications

Alternate Product Name: LIS1

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3-31

Epitope Target: LIS1

Epitope Species: human

Product Reactivity/Detection: LIS1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for LIS1 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin