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western blotting image from recommended IRF-4 (F-4) monoclonal antibody replacement. Image 398865 — このポリクローナル抗体は販売終了となりました。弊社推奨のIRF-4 (F-4)モノクローナル抗体をご覧ください(サンプルデータを表示)。

このポリクローナル抗体は販売終了となりました。弊社推奨のIRF-4 (F-4)モノクローナル抗体をご覧ください(サンプルデータを表示)。

IRF-4抗体(M-17): sc-6059

4.9(11)

レビューを書く質問する

IRF-4抗体 M-17はヤギポリクローナルIgGです。100 µg/mlで提供
エピトープはmouse由来のIRF-4のC-terminusに位置します
生産中止となったポリクローナル抗体

参考文献をチェックします (53)

クイックリンク

I was planning to purchase the "IRF-4 Antibody (M-17): sc-6059" for a CUT&RUN experiment, but I’ve discovered that it has been discontinued. Could you recommend any alternative antibodies that would be suitable for this application?

質問者: Ya J

Thank you for your question.We have not validated our antibodies for CUT&RUN experiments. Here is the alternative replacement : https://www.scbt.com/p/irf-4-antibody-f-4

回答者: BlakeJ

投稿日: 2024-08-12

Dear representative, is it possible to receive the datasheet of this product since we have it still in our lab. Additionally I would like to ask, which specific IgG subtype the antibody was (like IgG1, IgG2) Thank you

質問者: Naal

Thank you for your question. You can still download a PDF of the product data sheet on our website using the Datasheet Search at the bottom of our website under Resources (see link provided below). This is a goat polyclonal antibody and contains a mixture of IgG subtypes. https://www.scbt.com/scbt/resources/datasheet-search

回答者: Technical Support

投稿日: 2019-02-27

What is a starting dilution for IRF-4 to stain mouse tissues by IHC (paraffin embedded). Thanks.

質問者: Penny

Thank you for your question. We recommend a starting dilution of 1:50 (range of 1:50-1:500) for IHC(P). A link to our recommended protocol is below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Please note that this goat polyclonal antibody (sc-6059) is now discontinued. For a limited time, we are offering free 10µg sample sizes of our highly recommended mouse monoclonal replacement, IRF-4 Antibody (F-4): sc-48338. (https://www.scbt.com/scbt/product/irf-4-antibody-f-4). Please contact our Technical Service Department if you would like to test a sample.

回答者: Technical Support

投稿日: 2017-03-02

MHによって、 5つ星のうち 5と評価されています。 Used in WB and correct MW band at 1:200 dilutionUsed in WB and correct MW band at 1:200 dilution.

投稿日: 2015-07-01

JMによって、 5つ星のうち 5と評価されています。 Several publications describe success inSeveral publications describe success in Western Blot with this antibody using human transfected Jurkat cells, T-lymphocytes, KARPAS 299, K-562, U-937, 293T and B cells. -SCBT Publication Review

投稿日: 2015-04-22

SHによって、 5つ星のうち 4と評価されています。 A definite increase in signal can be observedA definite increase in signal can be observed in 293T cells that have been transfected to over-express the human IRF-4 protein when compared to non-transfected 293T cells by western blot. -SCBT QC

投稿日: 2015-04-21

SFによって、 5つ星のうち 5と評価されています。 Works well for ChIP application using humanWorks well for ChIP application using human and mouse T-cells, 3T3-L1 cells, RAW cells. -SCBT Publication Review

投稿日: 2015-04-11

LFによって、 5つ星のうち 5と評価されています。 Antibody scAntibody sc-6059 successfully used to show an increase in expression of IRF-4 when activated using the IRF-4 CRISPR Activation Plasmid by Western Blot. -SCBT QC

投稿日: 2015-04-08

JMによって、 5つ星のうち 5と評価されています。 Published data for coPublished data for co-immunoprecipitation using S194,RAMOS, RAJI, and BJAB,293,BOSC cells,NIH 3T3 cells. -SCBT Publication Review

投稿日: 2015-04-03

MSによって、 5つ星のうち 5と評価されています。 This antibody was reported to be successfulThis antibody was reported to be successful in EMSA application using human DLD-1, HT29-18N2, U937, Jurkat, Raji, HeLa, SB cells and mouse NIH/3T3, RAW 264.7 cells. -SCBT Publication Review

投稿日: 2015-03-25

MCによって、 5つ星のうち 5と評価されています。 Researchers have reported success in flowResearchers have reported success in flow cytometry using PT and BR cell lines, JEKO, HBL2, MINO, and REC1 PT cell lines, H929, OCI-LY19 cell lines. -SCBT Publication Review

投稿日: 2015-03-06

IRF-4抗体(M-17) のレビュー - ページ 2

IRF-4抗体(M-17)は、 11件のレビューによって、 5つ星のうち 4.9と評価されています。

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製品名	カタログ #	単位	価格	数量	お気に入り
IRF-4 抗体 (F-4)	sc-48338	200 µg/ml	$316.00
IRF-4 (F-4): m-IgG Fc BP-HRP Bundle	sc-525558	200 µg Ab; 10 µg BP	$354.00
IRF-4 (F-4): m-IgG_2a BP-HRP Bundle	sc-546314	200 µg Ab; 10 µg BP	$354.00
IRF-4 抗体 (F-4) X	sc-48338 X	200 µg/0.1 ml	$316.00
IRF-4 抗体 (F-4) AC	sc-48338 AC	500 µg/ml, 25% agarose	$416.00
IRF-4 抗体 (F-4) HRP	sc-48338 HRP	200 µg/ml	$316.00
IRF-4 抗体 (F-4) FITC	sc-48338 FITC	200 µg/ml	$330.00
IRF-4 抗体 (F-4) PE	sc-48338 PE	200 µg/ml	$343.00
IRF-4 抗体 (F-4) Alexa Fluor^® 488	sc-48338 AF488	200 µg/ml	$357.00
IRF-4 抗体 (F-4) Alexa Fluor^® 546	sc-48338 AF546	200 µg/ml	$357.00
IRF-4 抗体 (F-4) Alexa Fluor^® 594	sc-48338 AF594	200 µg/ml	$357.00
IRF-4 抗体 (F-4) Alexa Fluor^® 647	sc-48338 AF647	200 µg/ml	$357.00
IRF-4 抗体 (F-4) Alexa Fluor^® 680	sc-48338 AF680	200 µg/ml	$357.00
IRF-4 抗体 (F-4) Alexa Fluor^® 790	sc-48338 AF790	200 µg/ml	$357.00

IRF-4抗体(M-17): sc-6059

IRF-4 抗体 (F-4)

IRF-4 (F-4): m-IgG Fc BP-HRP Bundle

IRF-4 (F-4): m-IgG2a BP-HRP Bundle

IRF-4 抗体 (F-4) X

IRF-4 抗体 (F-4) AC

IRF-4 抗体 (F-4) HRP

IRF-4 抗体 (F-4) FITC

IRF-4 抗体 (F-4) PE

IRF-4 抗体 (F-4) Alexa Fluor® 488

IRF-4 抗体 (F-4) Alexa Fluor® 546

IRF-4 抗体 (F-4) Alexa Fluor® 594

IRF-4 抗体 (F-4) Alexa Fluor® 647

IRF-4 抗体 (F-4) Alexa Fluor® 680

IRF-4 抗体 (F-4) Alexa Fluor® 790

I was planning to purchase the &quot;IRF-4 Antibody (M-17): sc-6059&quot; for a CUT&amp;RUN experiment, but I&rsquo;ve discovered that it has been discontinued. Could you recommend any alternative antibodies that would be suitable for this application?

Dear representative, is it possible to receive the datasheet of this product since we have it still in our lab. Additionally I would like to ask, which specific IgG subtype the antibody was (like IgG1, IgG2) Thank you

What is a starting dilution for IRF-4 to stain mouse tissues by IHC (paraffin embedded). Thanks.

IRF-4 (F-4): m-IgG_2a BP-HRP Bundle

IRF-4 抗体 (F-4) Alexa Fluor^® 488

IRF-4 抗体 (F-4) Alexa Fluor^® 546

IRF-4 抗体 (F-4) Alexa Fluor^® 594

IRF-4 抗体 (F-4) Alexa Fluor^® 647

IRF-4 抗体 (F-4) Alexa Fluor^® 680

IRF-4 抗体 (F-4) Alexa Fluor^® 790

I was planning to purchase the "IRF-4 Antibody (M-17): sc-6059" for a CUT&RUN experiment, but I’ve discovered that it has been discontinued. Could you recommend any alternative antibodies that would be suitable for this application?