Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IRAK-1 CRISPR/Cas9 KO Plasmid (m): sc-421100
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IRAK-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IRAK-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IRAK-1 Antibody (F-4): sc-5288
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRAK-1 CRISPR/Cas9 KO Plasmid (m) | sc-421100 | 20 µg | $397.00 |
Overview
Irak1 encodes interleukin-1 receptor–associated kinase 1 (IRAK-1), a serine/threonine kinase that functions as a central signal transducer downstream of IL-1 receptor and Toll-like receptor engagement in mouse innate immune cells. Following receptor stimulation, IRAK-1 participates in MyD88-dependent signaling, coordinating the assembly of the IRAK–TRAF6 complex to promote activation of NF-κB and MAPK pathways and subsequent inflammatory gene expression. IRAK-1 also contributes to regulation of cytokine production, interferon responses, and cross-talk with ubiquitin-dependent signaling and inflammasome-related processes. Dysregulated IRAK-1 signaling is relevant to experimental models of chronic inflammation and autoimmunity, infection susceptibility, and inflammation-associated tumor microenvironment phenotypes.
IRAK-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Irak1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Irak1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Irak1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IRAK-1 protein expression.
This CRISPR knockout system enables efficient generation of Irak1-deficient cell models for investigation of IRAK-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Irak1 exon(s) critical for IRAK-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Irak1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IRAK-1 CRISPR/Cas9 KO Plasmid (m) and IRAK-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Irak1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IRAK-1 HDR Plasmid (m) and IRAK-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Irak1 homology arms to support homology-directed repair at defined Irak1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.