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western blotting image from recommended NFKBIA/IkB alpha (H-4) monoclonal antibody replacement. Image 354367 — このポリクローナル抗体は販売終了となりました。弊社推奨のNFKBIA/IkB alpha (H-4)モノクローナル抗体をご覧ください(サンプルデータを表示)。

このポリクローナル抗体は販売終了となりました。弊社推奨のNFKBIA/IkB alpha (H-4)モノクローナル抗体をご覧ください(サンプルデータを表示)。

IκB-α抗体(C-21): sc-371

4.8(30)

レビューを書く質問する

I kappa B- alpha抗体 C-21はウサギポリクローナルIgGです。100 µg/mlで提供; またはヤギIgGsc-371-G、200 µg/ml
エピトープはhuman由来のIκB-αのC-terminusに位置します
生産中止となったポリクローナル抗体

参考文献をチェックします (907)

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Hi, I've tried this antibody in WB using 30 ug of rat  liver cytoplasm lysate. I blocked nitrocellulose membrane with 10% skim milk for 1 h followed by overnight incubation with 1:100 primary antibody. Unfortunately I saw only a band at 90kDa.

質問者: dany58

Thanks for your feedback. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge. Please contact our Technical Service by phone or email or by Live Chat if you need support or additional information

回答者: SCBT Heidelberg Support

投稿日: 2018-11-27

hi, i keep getting two bands using this antibody. one band appears at around 37 kda and another one above 37 kda. could you explain what i can do to solve this problem? i'm using hacat (human keratinocyte cell line), 5% skim milk for blocking thank you

質問者: shiba

Thank you for your question about our rabbit polyclonal antibody, sc-371: IkB-alpha (C-21). The expected molecular weight for IkB-alpha is approximately 37 kDa. This protein may also be phosphorylated which is one possible explanation for why you may also be detecting an additional slightly higher molecular weight band in your samples. I would recommend including additional controls in your experiment to confirm if the additional band above 37 kDa is also IkB-alpha, and a result of the binding of the specific primary antibody. One suggestion is to treat your lysates with Lambda Phosphatase: sc-200312 (https://www.scbt.com/scbt/product/lambda-phosphatase?requestFrom=search) to release phosphate groups and observe the difference in detection by Western blot. I would also recommend to perform a secondary antibody control blot (without primary antibody) to confirm that there is not any non-specific binding of your secondary antibody alone. Please contact our Technical Service Department for additional support in troubleshooting this antibody in your research application. https://www.scbt.com/scbt/resources/technical-service

回答者: Technical Support

投稿日: 2018-12-18

EBV girlによって、 5つ星のうち 5と評価されています。 Good for western blotFor western blot, use at 1:1000 in 5% milk TBST, overnight incubation with rocking at 4 degrees C. Example of published result: PMID 29950426

投稿日: 2020-01-09

NatasaVによって、 5つ星のうち 5と評価されています。 IkB antibody in rat samplesIt is working very well in the rat samples without unspecific bands

投稿日: 2019-08-02

sun2019によって、 5つ星のうち 5と評価されています。 quality of anti-IkBa antibodyThis antibody's quality is very good, and works very well in our hand.

投稿日: 2019-01-15

KuanYHによって、 5つ星のうち 4と評価されています。 Nice primary antibodyThe primary antibody is available for Western blotting in mice sample.

投稿日: 2017-06-13

Eshuによって、 5つ星のうち 5と評価されています。 Works really wellI have used this antibody for nearly five years and it worked really well with bone-marrow derived dendritic cell extracts of BALB/c and C57BL/6 mice. There were no non-specific bands and the band intensity for the specific bands was also very good.

投稿日: 2017-06-06

Steventianによって、 5つ星のうち 4と評価されています。 Excellent experience.I bought this antobody for my current research project involved in the IkB-alpha protein detection by western blot assay for the mice kidney samples, this antibody works very well!

投稿日: 2017-02-12

Carmeによって、 5つ星のうち 5と評価されています。 Very clean immunoblots and high sensitivityThis is a great antibody for immunoblot analisis of endogenous IkBalpha. Very clean western blots and it can be diluted even 1:5000.

投稿日: 2016-12-21

Jing Hによって、 5つ星のうち 5と評価されています。 Great result in WBGreat result in WB using human and rat lungs tissue.

投稿日: 2016-11-04

IκB-α抗体(C-21) のレビュー - ページ 2

IκB-α抗体(C-21)は、 30件のレビューによって、 5つ星のうち 4.8と評価されています。

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製品名	カタログ #	単位	価格	数量	お気に入り
NFKBIA/IkB alpha 抗体 (H-4)	sc-1643	200 µg/ml	$316.00
NFKBIA/IkB alpha (H-4): m-IgG Fc BP-HRP Bundle	sc-528129	200 µg Ab; 10 µg BP	$354.00
NFKBIA/IkB alpha (H-4): m-IgGκ BP-HRP Bundle	sc-520432	200 µg Ab, 40 µg BP	$354.00
NFKBIA/IkB alpha 抗体 (H-4) AC	sc-1643 AC	500 µg/ml, 25% agarose	$416.00
NFKBIA/IkB alpha 抗体 (H-4) HRP	sc-1643 HRP	200 µg/ml	$316.00
NFKBIA/IkB alpha 抗体 (H-4) FITC	sc-1643 FITC	200 µg/ml	$330.00
NFKBIA/IkB alpha 抗体 (H-4) PE	sc-1643 PE	200 µg/ml	$343.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 488	sc-1643 AF488	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 546	sc-1643 AF546	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 594	sc-1643 AF594	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 647	sc-1643 AF647	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 680	sc-1643 AF680	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 790	sc-1643 AF790	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) TRITC	sc-1643 TRITC	200 µg/ml	$320.00

IκB-α抗体(C-21): sc-371

NFKBIA/IkB alpha 抗体 (H-4)

NFKBIA/IkB alpha (H-4): m-IgG Fc BP-HRP Bundle

NFKBIA/IkB alpha (H-4): m-IgGκ BP-HRP Bundle

NFKBIA/IkB alpha 抗体 (H-4) AC

NFKBIA/IkB alpha 抗体 (H-4) HRP

NFKBIA/IkB alpha 抗体 (H-4) FITC

NFKBIA/IkB alpha 抗体 (H-4) PE

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 488

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 546

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 594

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 647

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 680

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 790

NFKBIA/IkB alpha 抗体 (H-4) TRITC

Hi, I've tried this antibody in WB using 30 ug of rat &nbsp;liver cytoplasm lysate. I blocked nitrocellulose membrane with 10% skim milk for 1 h followed by overnight incubation with 1:100 primary antibody. Unfortunately I saw only a band at 90kDa.

hi, i keep getting two bands using this antibody. one band appears at around 37 kda and another one above 37 kda. could you explain what i can do to solve this problem? i'm using hacat (human keratinocyte cell line), 5% skim milk for blocking thank you

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 488

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 546

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 594

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 647

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 680

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 790

Hi, I've tried this antibody in WB using 30 ug of rat liver cytoplasm lysate. I blocked nitrocellulose membrane with 10% skim milk for 1 h followed by overnight incubation with 1:100 primary antibody. Unfortunately I saw only a band at 90kDa.