Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IGFBP2 CRISPR/Cas9 KO Plasmid (m): sc-421063
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IGFBP2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IGFBP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IGFBP2 Antibody (G-4): sc-515134
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGFBP2 CRISPR/Cas9 KO Plasmid (m) | sc-421063 | 20 µg | $397.00 |
Overview
Igfbp2 encodes insulin-like growth factor binding protein 2 (IGFBP2), a secreted modulator of IGF-I/IGF-II bioavailability that tunes IGF receptor signaling and downstream PI3K–AKT and MAPK pathways. By regulating growth factor sequestration and presentation, IGFBP2 influences cell proliferation, survival, migration, and extracellular matrix interactions during development and tissue remodeling. In mouse systems, altered Igfbp2 expression has been associated with metabolic regulation, adipose biology, and neuroinflammatory or injury-associated responses, making it a useful node for pathway-level studies. Its context-dependent roles in oncogenic signaling and microenvironmental remodeling also support mechanistic investigation in cancer-relevant models without implying clinical outcomes.
IGFBP2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Igfbp2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Igfbp2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Igfbp2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IGFBP2 protein expression.
This CRISPR knockout system enables efficient generation of Igfbp2-deficient cell models for investigation of IGFBP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Igfbp2 exon(s) critical for IGFBP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Igfbp2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IGFBP2 CRISPR/Cas9 KO Plasmid (m) and IGFBP2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Igfbp2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IGFBP2 HDR Plasmid (m) and IGFBP2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Igfbp2 homology arms to support homology-directed repair at defined Igfbp2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.