Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HNF-1α Double Nickase Plasmid (h): sc-400594-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HNF-1α Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HNF-1α Double Nickase Plasmid (h) and HNF-1α Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HNF1A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HNF-1α Antibody (F-7): sc-393925
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HNF-1α Double Nickase Plasmid (h) | sc-400594-NIC | 20 µg | $410.00 | |||
HNF-1α Double Nickase Plasmid (h2) | sc-400594-NIC-2 | 20 µg | $410.00 |
HNF1A encodes hepatocyte nuclear factor 1-alpha (HNF-1α), a homeodomain-containing transcription factor that binds promoter and enhancer elements to regulate gene programs in hepatocytes, pancreatic β cells, renal epithelium, and intestinal tissues. It coordinates pathways involved in glucose sensing and insulin secretion, lipid and bile acid metabolism, and epithelial differentiation, in part through transcriptional networks with HNF4A and other liver-enriched factors. Perturbation of HNF-1α activity alters expression of transporters and metabolic enzymes, impacting cellular homeostasis and lineage-specific gene expression. Genetic and functional variants in HNF1A are linked to monogenic and complex metabolic phenotypes, making it a widely used node for dissecting transcriptional control of metabolism.
HNF-1α Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HNF1A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HNF1A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HNF1A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HNF1A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.