Grid2ip Antibody (A-4): sc-390952

Grid2ip Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Grid2ip protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Grid2ip (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Grid2ip protein, also known as glutamate receptor, ionotropic, δ2 (Grid2 or GluR-δ2) interacting protein 1 or delphilin, serves as a crucial postsynaptic scaffolding protein that contains one formin homology 2 domain and two PDZ domains, which are essential for protein-protein interactions. Grid2ip is predominantly expressed in Purkinje cells of the cerebellum, where Grid2ip localizes specifically to parallel fiber synapses, facilitating interaction with the C-terminus of GluR-δ2. This interaction links GluR-δ2, a glutamate receptor integral to motor learning, cerebellar wiring, and synaptic plasticity, with various signaling molecules and the actin cytoskeleton. Alternative splicing of Grid2ip gene results in three isoforms: L-delphilin, S-delphilin (or delphilin-α), and delphilin-β, each exhibiting distinct expression patterns and protein interactions. Palmitoylation of delphilin-α enhances GluR-δ2 expression on the cell surface and promotes delphilin-α accumulation in dendritic spines, underscoring this modification′s importance in synaptic function and plasticity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Grid2ip Antibody (A-4) References:

1. Delphilin: a novel PDZ and formin homology domain-containing protein that synaptically colocalizes and interacts with glutamate receptor delta 2 subunit.  |  Miyagi, Y., et al. 2002. J Neurosci. 22: 803-14. PMID: 11826110
2. Identification and characterization of human GRID2IP gene and rat Grid2ip gene in silico.  |  Katoh, M. and Katoh, M. 2003. Int J Mol Med. 12: 1015-9. PMID: 14612983
3. Identification and characterization of human DIAPH3 gene in silico.  |  Katoh, M. and Katoh, M. 2004. Int J Mol Med. 13: 473-8. PMID: 14767582
4. Identification and characterization of a novel Delphilin variant with an alternative N-terminus.  |  Yamashita, T., et al. 2005. Brain Res Mol Brain Res. 141: 83-94. PMID: 16168524
5. Characterization of the delta2 glutamate receptor-binding protein delphilin: Splicing variants with differential palmitoylation and an additional PDZ domain.  |  Matsuda, K., et al. 2006. J Biol Chem. 281: 25577-87. PMID: 16835239
6. Binding of glutamate receptor delta2 to its scaffold protein, Delphilin, is regulated by PKA.  |  Sonoda, T., et al. 2006. Biochem Biophys Res Commun. 350: 748-52. PMID: 17027646
7. The extreme C-terminus of GluRdelta2 is essential for induction of long-term depression in cerebellar slices.  |  Kohda, K., et al. 2007. Eur J Neurosci. 25: 1357-62. PMID: 17425562
8. The synaptic scaffolding protein Delphilin interacts with monocarboxylate transporter 2.  |  Watanabe-Kaneko, K., et al. 2007. Neuroreport. 18: 489-93. PMID: 17496809
9. Enhancement of both long-term depression induction and optokinetic response adaptation in mice lacking delphilin.  |  Takeuchi, T., et al. 2008. PLoS One. 3: e2297. PMID: 18509461

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Grid2ip Antibody (A-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Grid2ip Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Grid2ip Antibody (A-4) Specifications

Alternate Product Name: Grid2ip

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 91-280

Epitope Target: Grid2ip

Epitope Species: mouse

Product Reactivity/Detection: Grid2ip

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Grid2ip Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin