Date published: 2025-9-8

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GFP抗体(1A5): sc-101536

4.5(8)
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データシート
  • GFP抗体 1A5はラットモノクローナルIgG1です。200 µg/mlで提供
  • 組換え体 GFP に対する
  • GFP and GFP fusion proteins WB, IP, IF と ELISAでの検出にはお勧めします
  • 現在、GFP Antibody (1A5)に適した二次検出試薬の同定はまだ完了していません。この研究は進めています。

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関連項目

緑色蛍光タンパク質(GFP)はもともと、クラゲのオワンクラゲの生物発光に関与するタンパク質として同定された。GFP cDNAは原核細胞で発現させると蛍光を発するが、外因性の基質や補因子を必要としないため、GFPは生体内での遺伝子発現やタンパク質の局在をモニターする有用なツールとなる。野生型GFPよりも強い蛍光を発するEGFPなど、いくつかのGFP変異体が開発されている。励起極大がシフトしているため、FACSや蛍光顕微鏡、二重標識の応用に有利である。GFPは融合タンパク質のタグとして発現ベクターに広く用いられており、GFPに融合した異種タンパク質の発現とモニタリングが可能である。

試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。

注文情報

製品名カタログ #単位価格数量お気に入り

GFP 抗体 (1A5)

sc-101536
200 µg/ml
$316.00

Does this antibody(GFP Antibody (1A5)) works for venus? If not, do you have any idea which GFP antibody here can recognize Venus?

質問者: Liu M
Thank you for your question. Please contact our Technical Service Department with the specific protein sequence for Venus, and we can perform sequence analysis of our GFP antibody antigens to see if any of them may also detect this protein. We do not have any data or citations for this GFP antibody specifically mentioning reactivity with Venus.
回答者: Technical Support
投稿日: 2020-03-03

Could you tell me which regions or amino acid of the GFP protein is targeted by this antibody?

質問者: jfoul78
Thank you for your question. GFP Antibody (1A5) is a rat monoclonal raised against recombinant GFP. Unfortunately, the exact sequence used as an immunogen is not known, nor is the epitope recognized by this specific clone. We do offer a broad variety of GFP antibodies where this information is available. For example, our GFP Antibody (C-2): sc-390394 is specific for an epitope mapping between amino acids 167-182 within an internal region of GFP of Aequorea victoria origin. Please contact our Technical Service Department for help in choosing the best antibody for your research needs. Please call us toll-free at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat.
回答者: Technical Support
投稿日: 2017-02-03

I would like to use GFP Antibody (1A5): sc-101536 for double staining with another mouse monoclonal primary antibody. Is this possible?

質問者: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-101536 is not available directly conjugated, but we have an alternative GFP antibody (sc-9996) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
回答者: Technical Support 7
投稿日: 2017-01-24

I am looking for a GFP antibody that was raised against the amino acid sequence 215-230 (RDHMVL LEFVTAAGIT). Do you have any antibody like this? Thanks!

質問者: Anette
Thank you for your question. Most of our GFP antibodies were raised against full length recombinant GFP, which includes this portion of the sequence that you have specified. However, the exact epitope recognized by each monoclonal antibody has not been determined. If you would be interested in testing a complimentary sample size of our mouse monoclonal antibody, GFP (B-2): sc-9996, to see if it would work for your purposes, please contact our Technical Service Department. Please call us directly at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat.
回答者: Technical Support
投稿日: 2017-01-19
  • y_2025, m_8, d_30, h_9CST
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によって、 5つ星のうち 1と評価されています。 Did not work in texted tissue samplesI got a free sample and tested it according to th eworking lab protocol.There was no signal in the blot.
投稿日: 2018-04-09
によって、 5つ星のうち 5と評価されています。 Positive WB and IFAntibody is positive for both westren blot and IF staining
投稿日: 2017-02-05
によって、 5つ星のうち 5と評価されています。 Reliable antibodyGood choice if you're looking for a reliable GFP antibody to use in WB and IF.
投稿日: 2017-01-11
によって、 5つ星のうち 5と評価されています。 Strong bands at 1:1000 dilutionStrong bands at 1:1000 dilution.
投稿日: 2015-09-10
によって、 5つ星のうち 5と評価されています。 Worked well in WBWorked well in WB, no complaints.
投稿日: 2015-05-27
によって、 5つ星のうち 5と評価されています。 Good antibody for western blot analysis ofGood antibody for western blot analysis of GFP expression in 293T cell lysate. -SCBT QC
投稿日: 2015-04-07
によって、 5つ星のうち 5と評価されています。 Work well for IF staining of COS cellsWork well for IF staining of COS cells. -SCBT QC
投稿日: 2015-02-01
によって、 5つ星のうち 5と評価されています。 Generated publishable data on Hela cellsGenerated publishable data on Hela cells with this antibody. -SCBT Publication Review
投稿日: 2013-11-23
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GFP抗体(1A5)は、 8件のレビューによって、 5つ星のうち 4.5と 評価されています。
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