GFP Antibody (15): sc-101525
- GFP Antibody (15) is a mouse monoclonal IgG1 κ, cited in 24 publications, provided at 200 µg/ml
- raised against recombinant GFP
- recommended for detection of GFP by IF and IHC(P)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GFP Antibody (15). This work is in progress.
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GFP Antibody (15) is a mouse monoclonal IgG1 kappa light chain antibody developed against recombinant Green Fluorescent Protein (GFP), also referred to as Aequorea victoria GFP or Emerald GFP in certain variants. GFP is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria, renowned for its ability to emit bright green fluorescence when exposed to blue to ultraviolet light. This fluorescence is a result of GFP′s distinctive β-barrel structure, which consists of 11 β-strands that form a stable barrel enclosing an internal α-helix housing the chromophore. The β-barrel not only stabilizes the chromophore but also protects it from quenching interactions, ensuring efficient and reliable fluorescence. Anti-GFP antibody (15) is optimized for use in immunofluorescence (IF) and immunohistochemistry with paraffin-embedded sections (IHCP), enabling precise detection and localization of GFP-tagged proteins in various biological samples. GFP monoclonal antibody (15) facilitates the study of protein expression, localization, and interactions in live cells and tissues, making it an essential tool for researchers in molecular and cellular biology. Anti-GFP antibody (15) supports accurate monitoring of gene expression and protein dynamics in diverse experimental contexts by targeting GFP, which is widely employed as a fusion tag due to its non-invasive fluorescent properties.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GFP Antibody (15) References:
- GFP associates with microfilaments in fixed cells. | Schmitz, HD. and Bereiter-Hahn, J. 2001. Histochem Cell Biol. 116: 89-94. PMID: 11479727
- Understanding the folding of GFP using biophysical techniques. | Jackson, SE., et al. 2006. Expert Rev Proteomics. 3: 545-59. PMID: 17078767
- Producing a Mammalian GFP Expression Vector Containing Neomycin Resistance Gene. | Izadi, M., et al. 2009. Avicenna J Med Biotechnol. 1: 33-6. PMID: 23407141
- A novel GFP reporter mouse reveals Mustn1 expression in adult regenerating skeletal muscle, activated satellite cells and differentiating myoblasts. | Krause, MP., et al. 2013. Acta Physiol (Oxf). 208: 180-90. PMID: 23506283
- Application of GFP imaging in cancer. | Hoffman, RM. 2015. Lab Invest. 95: 432-52. PMID: 25686095
- GFP as a marker for transient gene transfer and expression in Mycoplasma hyorhinis. | Ishag, HZ., et al. 2016. Springerplus. 5: 769. PMID: 27386255
- Salicylic acid interferes with GFP fluorescence in vivo. | de Jonge, J., et al. 2017. J Exp Bot. 68: 1689-1696. PMID: 28369601
- Overcoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in Caenorhabditis elegans. | Teuscher, AC. and Ewald, CY. 2018. Bio Protoc. 8: PMID: 30073182
- Measurement of the fluorescence lifetime of GFP in high refractive index levitated droplets using FLIM. | Davidson, NM., et al. 2020. Phys Chem Chem Phys. 22: 14704-14711. PMID: 32573569
- GFP illuminates the cytoskeleton. | Ludin, B. and Matus, A. 1998. Trends Cell Biol. 8: 72-7. PMID: 9695812
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GFP Antibody (15) | sc-101525 | 200 µg/ml | $322.00 |