γPAK Antibody (F-12): sc-133113
- γPAK Antibody (F-12) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-41 at the N-terminus of γPAK of human origin
- recommended for detection of γPAK p62 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for γPAK Antibody (F-12) for WB applications. These reagents are now offered in bundles with γPAK Antibody (F-12) (see ordering information below).
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γPAK Antibody (F-12) is a mouse monoclonal IgG1 antibody that detects γPAK in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). γPAK, a serine/threonine kinase, plays a crucial role in cellular signaling pathways by interacting with small GTPases Rac1 and Cdc42, which are pivotal in regulating various cellular processes, including cytoskeletal dynamics and cell motility. γPAK (F-12) antibody helps researchers study how γPAK modulates GTPase activity, leading to autophosphorylation and subsequent dissociation from the complex to engage downstream substrates like Mek kinase, a key player in the JNK signaling pathway. γPAK has eight autophosphorylation sites, including Ser 19, Ser 141, and Thr 402, with the phosphorylation of Ser 141 and Thr 402 being particularly correlated with activation. This regulatory mechanism is vital for maintaining proper cellular responses to external stimuli, highlighting γPAK′s significance in both normal physiology and potential pathological conditions. anti-γPAK antibody (F-12) serves as an essential tool for researchers exploring the intricate signaling networks involving this important kinase.
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γPAK Antibody (F-12) References:
- Phosphorylation of non-muscle myosin II regulatory light chain by p21-activated kinase (gamma-PAK). | Chew, TL., et al. 1998. J Muscle Res Cell Motil. 19: 839-54. PMID: 10047984
- Multisite autophosphorylation of p21-activated protein kinase gamma-PAK as a function of activation. | Gatti, A., et al. 1999. J Biol Chem. 274: 8022-8. PMID: 10075701
- p21-activated protein kinase gamma-PAK is activated by ionizing radiation and other DNA-damaging agents. Similarities and differences to alpha-PAK. | Roig, J. and Traugh, JA. 1999. J Biol Chem. 274: 31119-22. PMID: 10531298
- Substrates enhance autophosphorylation and activation of p21-activated protein kinase gamma-PAK in the absence of activation loop phosphorylation. | Jakobi, R., et al. 2000. Eur J Biochem. 267: 4414-21. PMID: 10880965
- Functional interaction between c-Abl and the p21-activated protein kinase gamma-PAK. | Roig, J., et al. 2000. Proc Natl Acad Sci U S A. 97: 14346-51. PMID: 11121037
- p21-activated protein kinase gamma-PAK suppresses programmed cell death of BALB3T3 fibroblasts. | Jakobi, R., et al. 2001. J Biol Chem. 276: 16624-34. PMID: 11278362
- Cytostatic p21 G protein-activated protein kinase gamma-PAK. | Roig, J. and Traugh, JA. 2001. Vitam Horm. 62: 167-98. PMID: 11345898
- Cdc42-independent activation and translocation of the cytostatic p21-activated protein kinase gamma-PAK by sphingosine. | Roig, J., et al. 2001. FEBS Lett. 507: 195-9. PMID: 11684097
- p21-activated protein kinase gamma-PAK in pituitary secretory granules phosphorylates prolactin. | Tuazon, PT., et al. 2002. FEBS Lett. 515: 84-8. PMID: 11943200
- Cleavage and activation of p21-activated protein kinase gamma-PAK by CPP32 (caspase 3). Effects of autophosphorylation on activity. | Walter, BN., et al. 1998. J Biol Chem. 273: 28733-9. PMID: 9786869
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
γPAK Antibody (F-12) | sc-133113 | 200 µg/ml | $322.00 | |||
γPAK Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-539849 | 200 µg Ab; 10 µg BP | $361.00 | |||
γPAK Antibody (F-12): m-IgG1 BP-HRP Bundle | sc-541747 | 200 µg Ab; 20 µg BP | $361.00 | |||
γPAK (F-12) Neutralizing Peptide | sc-133113 P | 100 µg/0.5 ml | $69.00 |