G alpha q抗体(10) mouse, rat, human と canine 由来のGα q WB, IP と IFでの検出にはお勧めします

Gα q抗体(10): sc-136181

Gα q抗体(10)は、 5件のレビューによって、 5つ星のうち 4.4と 評価されています。
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データシート

    • Gα q抗体 (10)はマウスモノクローナルIgG1 κです。200 µg/mlで提供
    • human由来のGα qのアミノ酸22-31に対応します
    • mouse, rat, human と canine 由来のGα q WB, IP と IFでの検出にはお勧めします
    • IP用のアガロース に共役での利用可能です。WB、IHC(P)とELISA用のHRP に共役での利用可能です。
    • Gα q (10): sc-136181無料の10 µgサンプルを受け取るには、ご連絡ください。
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I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

質問者: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
回答者: Technical Support 15
投稿日: 2017-01-25
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によって、 5つ星のうち 5 と評価されています。 Great results with Anti Gαq (10) sc-136181 Anti-Gαq (10) sc-136181 works great in Western blot. Different amounts of whole neuronal cell extracts were lysed in 25 mM Tris HCl (7.4), 150 mM NaCl, 1% glycerol, 1 mM EDTA, 1% NP-40 and blotted with 1:500 of anti-Gαq. A single band of 42 KDa was clearly visualized by chemiluminescence.
投稿日: 2019-10-03
によって、 5つ星のうち 4 と評価されています。 Specific G alpha q antibody I tested this antibody on HEK293 cells that were transfected with plasmids containing either G alpha 11 or G alpha q. The antibody was specific for G alpha q and had minimal cross-reaction with G alpha 11. However, in a dilution of 1:500 a relatively long (1min+) exposure time was needed, despite good transfection efficiency and high amounts of protein loaded), so the antibody might not work so well for endogenous expression.
投稿日: 2018-07-03
によって、 5つ星のうち 5 と評価されています。 excellent choice for Western blotting If you're looking for a strong antibody for detection G alpha q to use in Western blot, this is a great choice. Specific and reliable!
投稿日: 2017-01-09
によって、 5つ星のうち 4 と評価されています。 Worked well in CoIP in human embryonic kidney Worked well in CoIP in human embryonic kidney (HEK293) cells, -SCBT Publication Review
投稿日: 2015-01-02
によって、 5つ星のうち 4 と評価されています。 Good specificity in WB in human platelet extracts Good specificity in WB in human platelet extracts. -SCBT QC
投稿日: 2014-01-25
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