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G<sub>&alpha; i-1</sub>/GNAI1 Antibody (R4) - Western Blotting - Image 374318
Gα i-1 抗体 (R4): sc-13533. 大鼠大脑 (A), 小鼠大脑 (B) 和人类大脑皮层 (C) 组织提取物中 Gα i-1 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
Gα i-1 抗体 (R4): sc-13533. 大鼠大脑 (A), 小鼠大脑 (B) 和人类大脑皮层 (C) 组织提取物中 Gα i-1 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Gα i-1/GNAI1 抗体 (R4): sc-13533

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说明书
  • Gα i-1/GNAI1抗体(R4)是小鼠单克隆IgG2b κ, 在35篇文献中引用，规格为200 µg/ml
  • 免疫rat物种的Gα i-1
  • 抗-Gα i-1/GNAI1 抗体 (R4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和bovine 来源的 Gα i-1
  • 抗Gα i-1/GNAI1抗体（R4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Gα i-1/GNAI1 Antibody (R4) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Gα i-1/GNAI1 Antibody (R4) 打包提供（请参阅下面的订购信息）。
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関連項目

额外Heterotrimeric G Protein抗体，包括G α 11, G α 12, G α 13, G α i1, G α i2, G α i3, G α o, G α olf, G α q, G α q11, G α q14, G α q8, G α t, G α t1, G β和G β 1

Gα i-1抗体（R4）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠、人类和牛的Gα i-1。抗Gα i-1抗体（R4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Gα i-1作为异三聚体G蛋白家族的一员，在细胞信号传导中发挥着至关重要的作用，该家族负责将信号从细胞表面受体传递到细胞内效应器。具体而言，Gα i-1参与抑制腺苷酸环化酶活性，从而降低环状AMP水平，这对于调节神经递质释放、激素分泌和细胞生长等各种生理过程至关重要。Gα i-1与特定受体和下游效应物的相互作用可以调节多种信号通路，因此Gα i-1成为心血管健康、神经生物学和癌症等领域研究的重要目标。了解Gα i-1的功能和相互作用有助于深入了解疾病机理和潜在的治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Gα i-1/GNAI1 抗体 (R4) 参考文献:

  1. 免疫沉淀大鼠脑中高亲和力、嘌呤核苷酸敏感、可溶化的μ-阿片受体：G蛋白G（αo）、G（αi1）和G（αi3）的共沉淀。  |  Chalecka-Franaszek, E., et al. 2000. J Neurochem. 74: 1068-78. PMID: 10693938
  2. G蛋白α(i1)氨基末端螺旋与Gbetagamma亚基解离并激活后的构象变化。  |  Medkova, M., et al. 2002. Biochemistry. 41: 9962-72. PMID: 12146960
  3. 伽马（i）（1）的十四烷基氨基末端在溶液中伽马（i）（1）亚基的结构和功能中起着关键作用。  |  Preininger, AM., et al. 2003. Biochemistry. 42: 7931-41. PMID: 12834345
  4. G 蛋白 gamma 亚基的选择性组织分布，包括通过 cDNA 克隆发现的一种新形式的 gamma 亚基。  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
  5. 异三聚体 GTP 结合蛋白 beta 亚基的多样性：新型 beta 亚基 cDNA 的特征。  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
  6. Gustducin 是一种味觉细胞特异性 G 蛋白，与转导蛋白关系密切。  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
  7. 信号转导中 G 蛋白的多样性。  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
  8. G{alpha}i1蛋白的GDP-GTP交换过程取决于添加的洗涤剂的类型和浓度，会加速或减慢。  |  Kubota, M., et al. 2009. J Biochem. 146: 875-80. PMID: 19703944
  9. 核结合蛋白1是G{alpha}i1的一种钙调节鸟苷核苷酸解离抑制剂。  |  Kapoor, N., et al. 2010. J Biol Chem. 285: 31647-60. PMID: 20679342
  10. 三种刺激性 GTP 结合蛋白的生化特征。大, 小 Gs 和嗅觉特异性 G 蛋白 Golf。  |  Jones, DT., et al. 1990. J Biol Chem. 265: 2671-6. PMID: 2105931
  11. 人类鸟嘌呤核苷酸结合蛋白α i1亚基的基因在囊性纤维化基因座附近。  |  Bloch, DB., et al. 1988. Am J Hum Genet. 42: 884-8. PMID: 3130752
  12. 与 G beta gamma, 受体和效应器相互作用的 G alpha 亚基的结构元素。  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

订购信息

产品名称产品编号规格价格数量收藏夹

Gα i-1/GNAI1 抗体 (R4)

sc-13533
200 µg/ml
$322.00

Gα i-1/GNAI1 (R4): m-IgG Fc BP-HRP 套装

sc-528286
200 µg Ab; 10 µg BP
$361.00

Gα i-1/GNAI1 (R4): m-IgGκ BP-HRP 套装

sc-520625
200 µg Ab, 40 µg BP
$361.00

Gα i-1/GNAI1 (R4): m-IgG2b BP-HRP 套装

sc-550024
200 µg Ab; 10 µg BP
$361.00

Gα i-1/GNAI1 抗体 (R4) AC

sc-13533 AC
500 µg/ml, 25% agarose
$424.00

Gα i-1/GNAI1 抗体 (R4) HRP

sc-13533 HRP
200 µg/ml
$322.00

Gα i-1/GNAI1 抗体 (R4) FITC

sc-13533 FITC
200 µg/ml
$336.00

Gα i-1/GNAI1 抗体 (R4) PE

sc-13533 PE
200 µg/ml
$349.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 488

sc-13533 AF488
200 µg/ml
$364.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 546

sc-13533 AF546
200 µg/ml
$364.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 594

sc-13533 AF594
200 µg/ml
$364.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 647

sc-13533 AF647
200 µg/ml
$364.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 680

sc-13533 AF680
200 µg/ml
$364.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 790

sc-13533 AF790
200 µg/ml
$364.00

Has this antibody tested if it cross-react with the drosophila ortholog Gai. Thanks

Asked by: curious researcher
Thank you for your question.G&alpha; i-1 (R4) is recommended for mouse,rat. human and bovine. There is a 77%homology in drosophila. We only warranty if the homology is 95% or higher.
Answered by: BlakeJ
Date published: 2023-05-09

Is sc-13533 (G&alpha; i-1 -R4-) tested for cross-reactivity with G&alpha;0 and G&alpha;-i 2 &amp; 3 isoforms

Asked by: Miguelon
Thank you for your question. sc-13533 is specific for G&alpha; i-1, and should not cross-react with any other G&alpha; forms.
Answered by: Tech Service
Date published: 2020-02-11

In which region of G&alpha; i-1 does the antibody for G&alpha; i-1 (R4): sc-13533 recognize (epitope region)?

Asked by: Alex2323
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2018-12-21

Can G&alpha; i-1 (R4): sc-13533 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, G&alpha; i-1 (R4): sc-13533 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by leeeew from antibody works for us, detected purified Gα i-1This antibody works for us in western blot. Used dilution is 1:200, protein was detected at the corresponding position on the blot, the lowest amount we loaded in our experiment is 10ng
Date published: 2025-06-17
Rated 4 out of 5 by ph2497 from Works for IFI used this for IF staining brain sections and it works well
Date published: 2017-08-15
Rated 5 out of 5 by sunzh from Good operationThis antibody is suitable for many cell lines, I used it to detect Gα i-1, which was derived from WB mouse, and achieved good results.
Date published: 2017-05-23
Rated 5 out of 5 by Theresae from The catalystThe test catalyst, adenylate cyclase, each of a very broad range of receptors specifically detects extracellular stimuli, whereas the effectors used to generate one or more intracellular messengers are less
Date published: 2017-02-09
Rated 5 out of 5 by cjMara from Very popular choice for WB!This monoclonal G alpha i-1 Antibody (R4) is an excellent choice for Western blotting, IF and IHC(P). Published over a dozen times, especially popular in the literature for Western blotting applications.
Date published: 2017-02-07
Rated 5 out of 5 by Goosfu from Recommended useublished data for IP and WB in mouse melanocytes,great western blot results,strong signal with low background,also nice IHC data.
Date published: 2017-02-07
Rated 5 out of 5 by meetyi from Great antibody for WBThis antibody is really great. In the experiment, the analysis results of Western blot were very strong and clear, and the baseband is perfect.antibody diluted at 1:400.
Date published: 2017-01-26
Rated 5 out of 5 by Wuuzi from reliableIt worked for me from the first attempt with a 1:1000 dilution, this has worked very well in WB, it is predictable and reliable.
Date published: 2017-01-12
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Gα i-1/GNAI1 抗体 (R4) is rated 4.6 out of 5 by 13.
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