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western blotting image from recommended Estrogen Receptor alpha (F-10) monoclonal antibody replacement. Image 369721 — このポリクローナル抗体は販売終了となりました。弊社推奨のEstrogen Receptor alpha (F-10)モノクローナル抗体をご覧ください(サンプルデータを表示)。

このポリクローナル抗体は販売終了となりました。弊社推奨のEstrogen Receptor alpha (F-10)モノクローナル抗体をご覧ください(サンプルデータを表示)。

ERα抗体(HC-20): sc-543

4.5(22)

レビューを書く質問する

ER alpha抗体 HC-20はウサギポリクローナルIgGです。100 µg/mlで提供
エピトープはhuman由来のERαのC-terminusに位置します
生産中止となったポリクローナル抗体

参考文献をチェックします (235)

For a manuscript under revision I urgently need accurate information about the epitope used to generate the HC-20 anti-ERalpha polyclonal antibody. I have a text document stating that it is the last 50 amino acids, but find nothing online?

質問者: HelenaP

Thank you for your question. Please contact our technical service team directly for further information on this product.

回答者: Tech Support Europe

投稿日: 2022-12-02

Hi I was using sc-543x for my chip experiments. I have few experiments left to do with the same antibody. why have been this antibody discontinued? Thanks

質問者: prati

Thank you for your question. Yes, our rabbit polyclonal antibody, ERα (HC-20): sc-543, has been discontinued and is no longer available. Our highly recommended replacement monoclonal antibodies for ERα have also been validated for ChIP. Both clones, ERα (F-10): sc-8002 and ERα (D-12): sc-8005, have been cited in numerous publications for use in Chromatin Immunoprecipitation (ChIP) applications. Please contact our Technical Service Department if you would like to request a free 10 µg sample of these antibodies to test in your application. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.

回答者: Technical Support

投稿日: 2021-11-04

IBYMEによって、 5つ星のうち 5と評価されています。 WB ERWb for Estrogen receptor in MCF7 cells, 50ug of whole protein extract were used

投稿日: 2019-02-13

JuliMaymoによって、 5つ星のうち 4と評価されています。 ER-alpha antibody western blotIt is a good antibody for Er-alpha detection by Western blot . We used it in a 1:1000 dilution in human placenta samples and the results can be observed in this paper. It is an antibody of specific recognition.

投稿日: 2018-05-17

Mirazによって、 5つ星のうち 3と評価されています。 Used to detect ER alpha protein levelsThis antibody is very useful for detection of ER alpha protein levels in animal brain tissue. In particular, we used this antibody to detect protein levels in mytosol in hippocampus and prefrontal cortex of rats. In general, we are satisfied with the obtained results from our samples. Dilutions of Ab, up to 1:1000.

投稿日: 2017-11-23

haifengによって、 5つ星のうち 5と評価されています。 Good for WBI tested this antibody for western blot using MCF7 total protein. As seen in photo, I got a specific band of ERalpha.

投稿日: 2017-01-10

Elmarによって、 5つ星のうち 5と評価されています。 Published WB and ChIP data on HMLE and MCF10APublished WB and ChIP data on HMLE and MCF10A cells and on MEF

投稿日: 2016-06-24

Yingqiu_Xieによって、 5つ星のうち 5と評価されています。 It is very clearn for western blotIt is very clearn for western blot

投稿日: 2015-10-20

Angによって、 5つ星のうち 5と評価されています。 I have used this antibody to do CHIP assayI have used this antibody to do CHIP assay as many paper used. To be honest, it works quite well. What s more, it is much cheaper than the similar products of other company.

投稿日: 2015-10-15

Joyによって、 5つ星のうち 5と評価されています。 I used this in WB in mouse cells and works greatI used this in WB in mouse cells and works great, correct band seen at 1:500 dilution.

投稿日: 2015-10-07

ERα抗体(HC-20) のレビュー - ページ 2

ERα抗体(HC-20)は、 22件のレビューによって、 5つ星のうち 4.5と評価されています。

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製品名	カタログ #	単位	価格	数量	お気に入り
Estrogen Receptor alpha 抗体 (F-10)	sc-8002	200 µg/ml	$316.00
Estrogen Receptor alpha (F-10): m-IgG Fc BP-HRP Bundle	sc-528195	200 µg Ab; 10 µg BP	$354.00
Estrogen Receptor alpha (F-10): m-IgG_2a BP-HRP Bundle	sc-547044	200 µg Ab; 10 µg BP	$354.00
Estrogen Receptor alpha (F-10): m-IgGκ BP-HRP Bundle	sc-520517	200 µg Ab, 40 µg BP	$354.00
Estrogen Receptor alpha 抗体 (F-10) X	sc-8002 X	200 µg/0.1 ml	$316.00
Estrogen Receptor alpha 抗体 (F-10) AC	sc-8002 AC	500 µg/ml, 25% agarose	$416.00
Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor^® 546	sc-8002 AF546	200 µg/ml	$357.00
Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor^® 594	sc-8002 AF594	200 µg/ml	$357.00
Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor^® 680	sc-8002 AF680	200 µg/ml	$357.00
Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor^® 790	sc-8002 AF790	200 µg/ml	$357.00
Estrogen Receptor alpha (F-10) 中和ペプチド	sc-8002 P	100 µg/0.5 ml	$68.00

ERα抗体(HC-20): sc-543

Estrogen Receptor alpha 抗体 (F-10)

Estrogen Receptor alpha (F-10): m-IgG Fc BP-HRP Bundle

Estrogen Receptor alpha (F-10): m-IgG2a BP-HRP Bundle

Estrogen Receptor alpha (F-10): m-IgGκ BP-HRP Bundle

Estrogen Receptor alpha 抗体 (F-10) X

Estrogen Receptor alpha 抗体 (F-10) AC

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor® 546

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor® 594

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor® 680

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor® 790

Estrogen Receptor alpha (F-10) 中和ペプチド

For a manuscript under revision I urgently need accurate information about the epitope used to generate the HC-20 anti-ERalpha polyclonal antibody. I have a text document stating that it is the last 50 amino acids, but find nothing online?

Hi I was using sc-543x for my chip experiments. I have few experiments left to do with the same antibody. why have been this antibody discontinued? Thanks

Estrogen Receptor alpha (F-10): m-IgG_2a BP-HRP Bundle

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor^® 546

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor^® 594

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor^® 680

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor^® 790