Dynamin II Antibody (5E4C2F3): sc-81150

Dynamin II Antibody (5E4C2F3) is a mouse monoclonal IgG1 antibody that recognizes Dynamin II in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Dynamin II plays a crucial role in cellular processes, particularly in endocytosis, where anti-Dynamin II antibody (5E4C2F3) facilitates the scission of newly formed vesicles from the plasma membrane, thereby regulating the internalization of various receptors and signaling molecules. This function is vital for maintaining cellular homeostasis and communication, as Dynamin II influences synaptic transmission and the recycling of membrane components. Dynamin II (5E4C2F3) monoclonal antibody shows highest expression levels in the testis, which underscores its importance in reproductive biology. Dynamin II contains specific domains that enable interaction with various effectors, including microtubules and SH3 domain-containing proteins, which are essential for GTPase activity and rapid endocytic process. Dynamin II monoclonal antibody (5E4C2F3) coordinates these interactions for effective cellular signaling and response, making anti-Dynamin II antibody (5E4C2F3) an invaluable tool for researchers studying these intricate biological mechanisms.

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Dynamin II Antibody (5E4C2F3) References:

1. Dynamin II interacts with syndecan-4, a regulator of focal adhesion and stress-fiber formation.  |  Yoo, J., et al. 2005. Biochem Biophys Res Commun. 328: 424-31. PMID: 15694365
2. Complexes of syndapin II with dynamin II promote vesicle formation at the trans-Golgi network.  |  Kessels, MM., et al. 2006. J Cell Sci. 119: 1504-16. PMID: 16551695
3. Increased association of dynamin II with myosin II in ras transformed NIH3T3 cells.  |  Jeong, SJ., et al. 2006. Acta Biochim Biophys Sin (Shanghai). 38: 556-62. PMID: 16894478
4. Myristyl trimethyl ammonium bromide and octadecyl trimethyl ammonium bromide are surface-active small molecule dynamin inhibitors that block endocytosis mediated by dynamin I or dynamin II.  |  Quan, A., et al. 2007. Mol Pharmacol. 72: 1425-39. PMID: 17702890
5. Phosphorylation of dynamin II at serine-764 is associated with cytokinesis.  |  Chircop, M., et al. 2011. Biochim Biophys Acta. 1813: 1689-99. PMID: 21195118
6. Dynamin II function is required for EGF-mediated Stat3 activation but not Erk1/2 phosphorylation.  |  Luwor, RB., et al. 2012. Growth Factors. 30: 220-9. PMID: 22574813
7. Pharmacological inhibition of dynamin II reduces constitutive protein secretion from primary human macrophages.  |  Kockx, M., et al. 2014. PLoS One. 9: e111186. PMID: 25347775
8. Dynamin II is required for 17β-estradiol signaling and autophagy-based ERα degradation.  |  Totta, P., et al. 2016. Sci Rep. 6: 23727. PMID: 27009360
9. Ubiquitously expressed dynamin-II has a higher intrinsic GTPase activity and a greater propensity for self-assembly than neuronal dynamin-I.  |  Warnock, DE., et al. 1997. Mol Biol Cell. 8: 2553-62. PMID: 9398675
10. Perturbation of dynamin II with an amphiphysin SH3 domain increases GLUT4 glucose transporters at the plasma membrane in 3T3-L1 adipocytes. Dynamin II participates in GLUT4 endocytosis.  |  Volchuk, A., et al. 1998. J Biol Chem. 273: 8169-76. PMID: 9525921

The preferred Dynamin II antibody is Dynamin II Antibody (G-4): sc-166669.