CYP2F2 Antibody (F-9): sc-374540
- CYP2F2 Antibody (F-9) is a mouse monoclonal IgG2a κ CYP2F2 antibody, cited in 11 publications, provided at 200 µg/ml
- raised against amino acids 189-262 mapping within an internal region of CYP2F2 of mouse origin
- CYP2F2 Antibody (F-9) is recommended for detection of CYP2F2 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-CYP2F2 Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CYP2F2 Antibody (F-9) for WB applications. These reagents are now offered in bundles with CYP2F2 Antibody (F-9) (see ordering information below).
CYP2F2 Antibody (F-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects CYP2F2 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CYP2F2 (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CYP2F2, or cytochrome P450 2F2, is a membrane-bound enzyme predominantly located in the endoplasmic reticulum of Clara cells in the lungs, where CYP2F2 plays a crucial role in the metabolism of xenobiotics, particularly potentially harmful compounds like naphthalene. CYP2F2′s ability to catalyze the conversion of naphthalene into a glutathione-dihydronaphthalene conjugate is significant because CYP2F2 helps detoxify harmful substances, although CYP2F2 can also lead to cellular necrosis in cases of high exposure. The polymorphic nature of CYP2F2 may influence individual susceptibility to lung toxins, making CYP2F2 an important target for research into respiratory health and toxicology. As the murine homolog of human CYP2F1, CYP2F2 serves as a valuable model for understanding the metabolic pathways and risks associated with similar compounds in humans.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CYP2F2 Antibody (F-9) References:
- Cytochrome P450 expression and metabolic activation of cooked food mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in MCF10A breast epithelial cells. | Thomas, RD., et al. 2006. Chem Biol Interact. 160: 204-16. PMID: 16527260
- Naphthalene toxicity in mice and aryl hydrocarbon receptor-mediated CYPs. | Genter, MB., et al. 2006. Biochem Biophys Res Commun. 348: 120-3. PMID: 16876762
- The cytochromes P450 (CYP) response to allergic inflammation of the lung. | Stoilov, I., et al. 2006. Arch Biochem Biophys. 456: 30-8. PMID: 17081494
- Lithium toxicity and expression of stress-related genes or proteins in A549 cells. | Allagui, MS., et al. 2007. Biochim Biophys Acta. 1773: 1107-15. PMID: 17512992
- Reverse transcriptase-PCR quantification of mRNA levels from cytochrome (CYP)1, CYP2 and CYP3 families in 22 different human tissues. | Bièche, I., et al. 2007. Pharmacogenet Genomics. 17: 731-42. PMID: 17700362
- Mechanism-based inactivation of lung-selective cytochrome P450 CYP2F enzymes. | Kartha, JS. and Yost, GS. 2008. Drug Metab Dispos. 36: 155-62. PMID: 17962375
- Human drug metabolism genes in parathion-and estrogen-treated breast cells. | Calaf, GM. and Roy, D. 2007. Int J Mol Med. 20: 875-81. PMID: 17982697
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP2F2 Antibody (F-9) | sc-374540 | 200 µg/ml | $322.00 | |||
CYP2F2 Antibody (F-9): m-IgG Fc BP-HRP Bundle | sc-529765 | 200 µg Ab; 10 µg BP | $361.00 | |||
CYP2F2 Antibody (F-9): m-IgGκ BP-HRP Bundle | sc-522771 | 200 µg Ab, 40 µg BP | $361.00 | |||
CYP2F2 Antibody (F-9): m-IgG2a BP-HRP Bundle | sc-547375 | 200 µg Ab; 10 µg BP | $361.00 | |||
CYP2F2 Antibody (F-9) AC | sc-374540 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CYP2F2 Antibody (F-9) HRP | sc-374540 HRP | 200 µg/ml | $322.00 | |||
CYP2F2 Antibody (F-9) FITC | sc-374540 FITC | 200 µg/ml | $336.00 | |||
CYP2F2 Antibody (F-9) PE | sc-374540 PE | 200 µg/ml | $349.00 | |||
CYP2F2 Antibody (F-9) Alexa Fluor® 488 | sc-374540 AF488 | 200 µg/ml | $364.00 | |||
CYP2F2 Antibody (F-9) Alexa Fluor® 546 | sc-374540 AF546 | 200 µg/ml | $364.00 | |||
CYP2F2 Antibody (F-9) Alexa Fluor® 594 | sc-374540 AF594 | 200 µg/ml | $364.00 | |||
CYP2F2 Antibody (F-9) Alexa Fluor® 647 | sc-374540 AF647 | 200 µg/ml | $364.00 | |||
CYP2F2 Antibody (F-9) Alexa Fluor® 680 | sc-374540 AF680 | 200 µg/ml | $364.00 | |||
CYP2F2 Antibody (F-9) Alexa Fluor® 790 | sc-374540 AF790 | 200 µg/ml | $364.00 |