CSN8 Double Nickase Plasmid (h): sc-409800-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CSN8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CSN8 Double Nickase Plasmid (h) and CSN8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COPS8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CSN8 Antibody (F-8): sc-393482
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CSN8 Double Nickase Plasmid (h) | sc-409800-NIC | 20 µg | $410.00 | |||
CSN8 Double Nickase Plasmid (h2) | sc-409800-NIC-2 | 20 µg | $410.00 |
COPS8 encodes CSN8, a core component of the COP9 signalosome (CSN) that regulates cullin-RING E3 ubiquitin ligases through control of cullin neddylation/deneddylation dynamics. By modulating ubiquitin-dependent proteostasis, CSN8 influences cell-cycle progression, DNA damage responses, and signal transduction pathways that depend on timely turnover of key regulatory proteins. CSN8 function intersects with ubiquitin–proteasome system activity and stress-adaptive programs such as autophagy, shaping cellular homeostasis across proliferative and differentiated states. Dysregulation of COP9 signalosome subunits, including altered COPS8 expression or perturbation of CSN integrity, has been associated with abnormal growth control and disease-relevant changes in proteome stability in human cells.
CSN8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COPS8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COPS8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COPS8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COPS8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.