Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CPEB Double Nickase Plasmid (h): sc-402685-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CPEB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CPEB Double Nickase Plasmid (h) and CPEB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CPEB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CPEB Antibody (G-6): sc-514688
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPEB Double Nickase Plasmid (h) | sc-402685-NIC | 20 µg | $410.00 | |||
CPEB Double Nickase Plasmid (h2) | sc-402685-NIC-2 | 20 µg | $410.00 |
CPEB1 encodes cytoplasmic polyadenylation element binding protein (CPEB), an RNA-binding regulator that controls mRNA poly(A) tail length and translational timing through recognition of cytoplasmic polyadenylation elements in 3′ UTRs. By coordinating polyadenylation-dependent translation, CPEB1 contributes to post-transcriptional gene regulation programs that shape cell-cycle progression, differentiation, and neuronal synaptic plasticity. CPEB1 activity interfaces with signaling pathways that modulate RNA granule dynamics and local translation, influencing proteome remodeling in response to developmental and stress cues. Dysregulated CPEB1-mediated translational control has been associated with altered proliferation and differentiation states and has been studied in the context of cancer biology and neurobiology where RNA regulation is frequently perturbed.
CPEB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CPEB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CPEB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CPEB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CPEB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.