CAF-1 p150 Antibody (C-5): sc-133068

CAF-1 p150 Antibody (C-5) is a mouse monoclonal IgG1 antibody that detects chromatin assembly factor-1 p150 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CAF-1 p150 is a crucial component of the chromatin assembly factor-1 complex, which plays a vital role in the deposition of newly synthesized and acetylated histones H3 and H4 into nascent chromatin during DNA replication. This process is essential for maintaining the structural integrity of the genome, as DNA is properly packaged into chromatin, allowing for accurate replication and gene expression. The p150 subunit is particularly important for the maintenance of heterochromatin, which is characterized by its densely coiled structure and low gene density, making heterochromatin relatively inaccessible to DNA-modifying agents. During late S phase, p150 interacts with heterochromatin-associated proteins, such as HP1α, HP1β, and HP1γ, facilitating the orderly assembly of chromatin. As cells transition into mitosis, p150 and HP1 proteins dissociate from heterochromatin alongside the phosphorylation of histone H3, highlighting the dynamic nature of chromatin organization. CAF-1 p150′s ability to reassociate with chromatin post-mitosis, as histone H3 is dephosphorylated, underscores p150′s critical role in the epigenetic regulation of gene expression and chromatin structure.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CAF-1 p150 Antibody (C-5) References:

1. Molecular determinants for targeting heterochromatin protein 1-mediated gene silencing: direct chromoshadow domain-KAP-1 corepressor interaction is essential.  |  Lechner, MS., et al. 2000. Mol Cell Biol. 20: 6449-65. PMID: 10938122
2. A truncated form of the human CAF-1 p150 subunit impairs the maintenance of transcriptional gene silencing in mammalian cells.  |  Tchénio, T., et al. 2001. Mol Cell Biol. 21: 1953-61. PMID: 11238931
3. The mammalian heterochromatin protein 1 binds diverse nuclear proteins through a common motif that targets the chromoshadow domain.  |  Lechner, MS., et al. 2005. Biochem Biophys Res Commun. 331: 929-37. PMID: 15882967
4. The replication kinase Cdc7-Dbf4 promotes the interaction of the p150 subunit of chromatin assembly factor 1 with proliferating cell nuclear antigen.  |  Gérard, A., et al. 2006. EMBO Rep. 7: 817-23. PMID: 16826239
5. Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites.  |  Zhu, Q., et al. 2009. DNA Repair (Amst). 8: 262-73. PMID: 19059499
6. Two fundamentally distinct PCNA interaction peptides contribute to chromatin assembly factor 1 function.  |  Rolef Ben-Shahar, T., et al. 2009. Mol Cell Biol. 29: 6353-65. PMID: 19822659
7. Identification of a functional network of human epigenetic silencing factors.  |  Poleshko, A., et al. 2010. J Biol Chem. 285: 422-33. PMID: 19880521
8. An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins.  |  Hoek, M., et al. 2011. J Biol Chem. 286: 10876-87. PMID: 21209461
9. Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4.  |  Verreault, A., et al. 1996. Cell. 87: 95-104. PMID: 8858152
10. Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle.  |  Marheineke, K. and Krude, T. 1998. J Biol Chem. 273: 15279-86. PMID: 9614144

The preferred CAF-1 p150 antibody is CAF-1 p150 Antibody (D-1): sc-133105.