CAF-1 p150 Antibody (C-3): sc-137141

CAF-1 p150 Antibody (C-3) is a mouse monoclonal IgG2b antibody that detects chromatin assembly factor-1 p150 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CAF-1 p150 is a crucial component of the chromatin assembly factor-1 complex, which plays a vital role in the deposition of newly synthesized and acetylated histones H3 and H4 into nascent chromatin during DNA replication. This process is essential for maintaining the structural integrity of the genome, as DNA is properly packaged into chromatin, allowing for accurate replication and gene expression. The p150 subunit is particularly important for the maintenance of heterochromatin, a tightly packed form of DNA that is generally transcriptionally inactive and replicates late in the S phase of the cell cycle. Heterochromatin is characterized by low gene density and the presence of repetitive DNA sequences, which can be challenging for cellular machinery to access. During late S phase, p150 interacts directly with heterochromatin-associated proteins, such as HP1α, HP1β, and HP1γ, facilitating the orderly assembly of chromatin. As cells transition into mitosis, the dissociation of CAF-1 p150 and HP1 proteins from heterochromatin occurs alongside the phosphorylation of histone H3, a modification that is critical for the regulation of chromatin dynamics. The reassociation of HP1 proteins with chromatin at the end of mitosis, following the dephosphorylation of histone H3, underscores the importance of CAF-1 p150 in the cell cycle and chromatin organization.

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CAF-1 p150 Antibody (C-3) References:

1. Molecular determinants for targeting heterochromatin protein 1-mediated gene silencing: direct chromoshadow domain-KAP-1 corepressor interaction is essential.  |  Lechner, MS., et al. 2000. Mol Cell Biol. 20: 6449-65. PMID: 10938122
2. Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene.  |  Dong, H., et al. 2001. Gene. 264: 187-96. PMID: 11250073
3. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1.  |  Reese, BE., et al. 2003. Mol Cell Biol. 23: 3226-36. PMID: 12697822
4. The mammalian heterochromatin protein 1 binds diverse nuclear proteins through a common motif that targets the chromoshadow domain.  |  Lechner, MS., et al. 2005. Biochem Biophys Res Commun. 331: 929-37. PMID: 15882967
5. Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites.  |  Zhu, Q., et al. 2009. DNA Repair (Amst). 8: 262-73. PMID: 19059499
6. Identification of a functional network of human epigenetic silencing factors.  |  Poleshko, A., et al. 2010. J Biol Chem. 285: 422-33. PMID: 19880521
7. An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins.  |  Hoek, M., et al. 2011. J Biol Chem. 286: 10876-87. PMID: 21209461
8. Mapping of the gene for the p60 subunit of the human chromatin assembly factor (CAF1A) to the Down syndrome region of chromosome 21.  |  Blouin, JL., et al. 1996. Genomics. 33: 309-12. PMID: 8660983
9. Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4.  |  Verreault, A., et al. 1996. Cell. 87: 95-104. PMID: 8858152
10. Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle.  |  Marheineke, K. and Krude, T. 1998. J Biol Chem. 273: 15279-86. PMID: 9614144

The preferred CAF-1 p150 antibody is CAF-1 p150 Antibody (D-1): sc-133105.