Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
C23 (Nucleolin) CRISPR/Cas9 KO Plasmid (m): sc-421835
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C23 (Nucleolin) CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C23 (Nucleolin) genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: C23 Antibody (MS-3): sc-8031
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C23 (Nucleolin) CRISPR/Cas9 KO Plasmid (m) | sc-421835 | 20 µg | $397.00 |
Overview
Ncl encodes C23 (nucleolin), an abundant nucleolar phosphoprotein that coordinates rRNA transcription, pre-rRNA processing, and ribosome assembly, supporting global proteostasis and cell growth. Nucleolin also shuttles between the nucleus, cytoplasm, and cell surface to influence chromatin organization, DNA damage responses, mRNA stability, and signaling outputs linked to stress adaptation. Through interactions with RNA polymerase I machinery, ribonucleoprotein complexes, and key cell-cycle regulators, C23 integrates nucleolar function with proliferation and apoptosis pathways. Dysregulated nucleolin expression or localization has been associated with altered ribosome biogenesis, aberrant translation programs, and oncogenic phenotypes, making it a frequent focus in studies of tumor biology and cellular stress.
C23 (Nucleolin) CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ncl gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ncl together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ncl open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C23 (Nucleolin) protein expression.
This CRISPR knockout system enables efficient generation of Ncl-deficient cell models for investigation of C23 (Nucleolin) signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ncl exon(s) critical for C23 (Nucleolin) function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ncl genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C23 (Nucleolin) CRISPR/Cas9 KO Plasmid (m) and C23 (Nucleolin) CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ncl locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C23 (Nucleolin) HDR Plasmid (m) and C23 (Nucleolin) HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ncl homology arms to support homology-directed repair at defined Ncl target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.