β-glucosidase (h): 293T Lysate: sc-110483

β-Glucosidase (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line modified to express the SV40 large T antigen, which enhances its transfection efficiency and protein production capabilities. This lysate is enriched with human β-glucosidase, an enzyme that catalyzes the hydrolysis of β-glucosidic bonds in glucosides and oligosaccharides, playing a crucial role in carbohydrate metabolism. β-Glucosidase is essential for the breakdown of complex carbohydrates into simpler sugars, which can then be utilized by the cell for energy production and other metabolic processes. In research, β-Glucosidase (h): 293T Lysate is used to study the enzyme's activity, structure, and regulatory mechanisms. Researchers employ this lysate in enzymatic activity assays to measure the hydrolysis rates of β-glucosidic substrates, providing insights into the kinetic properties and efficiency of β-glucosidase. This lysate is also valuable in Western blotting to detect and quantify β-glucosidase expression under various experimental conditions, facilitating the study of its regulation and expression patterns. Additionally, β-Glucosidase (h): 293T Lysate is utilized in co-immunoprecipitation assays to identify interacting partners and to understand the protein networks involved in carbohydrate metabolism. Structural studies using this lysate help explain the three-dimensional conformation of β-glucosidase, revealing how substrate binding and catalysis occur at the molecular level.