ZDHHC9 Double Nickase Plasmid (m): sc-431594-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZDHHC9 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZDHHC9 Double Nickase Plasmid (m) and ZDHHC9 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Zdhhc9. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZDHHC9 Double Nickase Plasmid (m) | sc-431594-NIC | 20 µg | $410.00 |
Zdhhc9 encodes ZDHHC9, a DHHC-family palmitoyltransferase that catalyzes S-palmitoylation of protein substrates to regulate membrane association, trafficking, and signal compartmentalization. By modulating lipidation-dependent localization of signaling proteins, ZDHHC9 contributes to pathways controlling vesicle dynamics and cell communication, with downstream effects on growth and differentiation programs. In mouse systems, altered DHHC-mediated palmitoylation has been linked to neurodevelopmental phenotypes and dysregulated signaling networks, making Zdhhc9 a useful node for studying how post-translational lipidation shapes cellular function. Its activity is often examined alongside other palmitoylation machinery and membrane microdomain organization to connect substrate modification with pathway output.
ZDHHC9 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Zdhhc9 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Zdhhc9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Zdhhc9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Zdhhc9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.