ZBP1 Double Nickase Plasmid (h): sc-406434-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZBP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZBP1 Double Nickase Plasmid (h) and ZBP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZBP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZBP1 Antibody (H-9): sc-271483
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZBP1 Double Nickase Plasmid (h) | sc-406434-NIC | 20 µg | $410.00 | |||
ZBP1 Double Nickase Plasmid (h2) | sc-406434-NIC-2 | 20 µg | $410.00 |
ZBP1 (Z-DNA binding protein 1; also known as DAI) is an innate immune sensor that recognizes Z-form nucleic acids and couples nucleic acid detection to inflammatory and cell death programs. Through RHIM-dependent interactions with RIPK3 and other signaling partners, ZBP1 can promote necroptotic and apoptotic responses and modulate interferon-stimulated gene expression downstream of pathogen sensing. It is integrated into antiviral defense pathways and contributes to regulation of NF-κB and type I interferon signaling in response to infection or endogenous nucleic acid stress. Dysregulated ZBP1 activity has been linked to aberrant inflammation and immune-mediated pathology, making it relevant for studying mechanisms of inflammatory disease and host–pathogen interactions.
ZBP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZBP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZBP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZBP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZBP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.