Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
XRCC4 Double Nickase Plasmid (m): sc-430821-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XRCC4 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- XRCC4 Double Nickase Plasmid (m) and XRCC4 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Xrcc4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XRCC4 Antibody (G-10): sc-365118
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XRCC4 Double Nickase Plasmid (m) | sc-430821-NIC | 20 µg | $410.00 |
Mouse Xrcc4 encodes XRCC4, a core scaffold protein in the non-homologous end joining (NHEJ) pathway that repairs DNA double-strand breaks generated by ionizing radiation, oxidative stress, and programmed recombination events. XRCC4 forms a functional complex with DNA ligase IV and interacts with XLF/NHEJ1 to align and ligate broken DNA ends, thereby preserving genome stability and supporting proper cell cycle progression. Disruption of XRCC4-dependent end joining compromises chromosomal integrity and increases sensitivity to genotoxic stress, linking XRCC4 function to mechanisms underlying cancer-associated genomic instability. Xrcc4 is also relevant to studies of immune system development because NHEJ is required for V(D)J recombination and diversification of antigen receptor genes.
XRCC4 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Xrcc4 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Xrcc4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Xrcc4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Xrcc4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.