VAX1 CRISPR/Cas9 KO Plasmid (m): sc-423657
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VAX1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VAX1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VAX1 CRISPR/Cas9 KO Plasmid (m) | sc-423657 | 20 µg | $397.00 |
Overview
Vax1 encodes VAX1, a homeobox transcription factor required for embryonic patterning of the forebrain and eye, with prominent roles in ventral telencephalon and optic stalk development. VAX1 regulates gene expression programs that coordinate neural progenitor specification, axon guidance, and midline formation, integrating with developmental signaling pathways such as SHH-mediated ventralization and other homeobox networks. Mouse genetic studies link Vax1 dysfunction to congenital craniofacial and ocular phenotypes, including microphthalmia/anophthalmia and abnormalities of the hypothalamic–pituitary region. These properties make VAX1 a useful node for dissecting transcriptional control of neurodevelopmental trajectories and lineage decisions.
VAX1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Vax1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Vax1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Vax1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VAX1 protein expression.
This CRISPR knockout system enables efficient generation of Vax1-deficient cell models for investigation of VAX1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Vax1 exon(s) critical for VAX1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Vax1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VAX1 CRISPR/Cas9 KO Plasmid (m) and VAX1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Vax1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VAX1 HDR Plasmid (m) and VAX1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Vax1 homology arms to support homology-directed repair at defined Vax1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.