USP2 CRISPR/Cas9 KO Plasmid (m): sc-424700
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the USP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP2 CRISPR/Cas9 KO Plasmid (m) | sc-424700 | 20 µg | $397.00 |
Overview
Usp2 encodes ubiquitin-specific peptidase 2 (USP2), a deubiquitinating enzyme that reverses ubiquitin-dependent protein turnover to shape proteostasis and signal duration. By trimming ubiquitin chains on selected substrates, USP2 modulates processes such as cell-cycle progression, apoptosis, DNA damage responses, and inflammatory signaling, and it is linked to regulation of metabolic and circadian programs. Altered USP2 activity has been associated with dysregulated growth and stress-adaptation phenotypes in experimental models, making it a useful node for interrogating ubiquitin–proteasome system control of cellular homeostasis. In mouse systems, Usp2 perturbation is commonly used to study how deubiquitination tunes pathway crosstalk in tissues and immune-relevant contexts.
USP2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Usp2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Usp2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Usp2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish USP2 protein expression.
This CRISPR knockout system enables efficient generation of Usp2-deficient cell models for investigation of USP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Usp2 exon(s) critical for USP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Usp2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by USP2 CRISPR/Cas9 KO Plasmid (m) and USP2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Usp2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by USP2 HDR Plasmid (m) and USP2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Usp2 homology arms to support homology-directed repair at defined Usp2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.