Anticorpo Troponin I-C (C5) raccomandato per il rilevamento di Troponin I, cardiac muscle di origine mouse, rat e human in WB, IP, IF e IHC(P)

Anticorpo Troponin I-C (C5): sc-33728

Anticorpo Troponin I-C (C5) valutazione 4.3 di 5 di 3.
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Schede tecniche

    • Troponin I-C Antibody C5 è un monoclonale di topo IgG2b fornito in 100 µg/ml
    • raised against free human cardiac Troponin and/or native Troponin complex, with epitope mapping to amino acids 186-192
    • raccomandato per il rilevamento di Troponin I, cardiac muscle di origine mouse, rat e human in WB, IP, IF e IHC(P); potrebbe cross-reagire con skeletal muscle Troponin I
    • Vedere Troponin I (E-9): sc-365446 for Troponin I-C per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Troponin I-C Antibody (C5). This work is in progress.
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Can sc-33728: Troponin I-C (C5) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Richiesta da: AbPolly
Thank you for your question. Yes, sc-33728: Troponin I-C (C5) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-03-24
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Rated 5 di 5 di da worked worked
Data di pubblicazione: 2015-05-28
Rated 4 di 5 di da Strong band in WB detected using mouse heart Strong band in WB detected using mouse heart tissue extract. -SCBT QC
Data di pubblicazione: 2015-05-16
Rated 4 di 5 di da Produced wonderful immunoperoxidase cytoplasmic Produced wonderful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human heart muscle tissue. -SCBT QC
Data di pubblicazione: 2015-03-20
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