Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TPX2 CRISPR/Cas9 KO Plasmid (h): sc-402581
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TPX2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TPX2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TPX2 Antibody (E-2): sc-271570
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TPX2 CRISPR/Cas9 KO Plasmid (h) | sc-402581 | 20 µg | $397.00 |
Overview
TPX2 (targeting protein for Xklp2) encodes a microtubule-associated protein that is essential for mitotic spindle assembly and maintenance, including Ran-GTP–dependent microtubule nucleation around chromosomes and activation/localization of Aurora A kinase at spindle poles. TPX2 supports centrosome integrity, kinetochore fiber formation, and accurate chromosome segregation, linking it to cell-cycle progression and genomic stability pathways. Dysregulated TPX2 expression is frequently associated with proliferative phenotypes and chromosomal instability in cancer-related contexts, making it a widely used marker and mechanistic node in mitotic signaling studies. Human TPX2 is therefore relevant for investigating spindle checkpoint function, aneuploidy, and mitosis-driven vulnerability mechanisms in biomedical research models.
TPX2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TPX2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TPX2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TPX2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TPX2 protein expression.
This CRISPR knockout system enables efficient generation of TPX2-deficient cell models for investigation of TPX2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TPX2 exon(s) critical for TPX2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TPX2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TPX2 CRISPR/Cas9 KO Plasmid (h) and TPX2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TPX2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TPX2 HDR Plasmid (h) and TPX2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TPX2 homology arms to support homology-directed repair at defined TPX2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.