Anticorpo TCR delta (H-41) raccomandato per il rilevamento di TCR δ di origine human in WB, IP e ELISA

Anticorpo TCR δ (H-41): sc-100289

Anticorpo TCR δ (H-41) valutazione 4.6 di 5 di 5.
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Schede tecniche

    • Anti-TCR delta Antibody H-41 è un monoclonale di topo IgG1 (kappa light chain) TCR δ antibody fornito in 100 µg/ml
    • generato contro il ricombinante TCR δ di origine human
    • raccomandato per il rilevamento di TCR δ di origine human in WB, IP e ELISA
    • Vedi m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), le nostre alternative ricombinanti ai reagenti secondari convenzionali anti-mouse IgG altamente raccomandate.
    • Contattaci per ricevere un FREE 10 µg sample di TCR δ (H-41): sc-100289.

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Richiesta da: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Risposta da: Technical Service 11
Data di pubblicazione: 2017-02-03
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Rated 5 di 5 di da Very good TCRgd+ cell staining on FFPE After TCR gamma.20 (from Thermo Scientific) being discontinued, finally I've found in Santa Cruz Biotechnology TCR delta a solid antibody useful to recognize TCRgd cells in formalin-fixed paraffin embedded sample using immunohistochemistry. I've stained sections from tonsil and small intestine with a range of dilution from 1:25 to 1:250 and I've achieved strong and tidy stainings in all dilutions tested. Also antigen unmasking is very tolerant. It works using EDTA pH8.0 or Citrate pH 6.0 in microwave or water bath. I'm very satisfied from the results
Data di pubblicazione: 2020-03-12
Rated 5 di 5 di da Excellent staining for IHC on FFPE tissues I successfully used this product for immunohistochemistry on 4 micron sections of FFPE tonsil tissues and FFPE cell block of a bone marrow blood clot. I got excellent results using 1:50-1:100 dilution for 1 hour incubation at room temperature manually or using the Ventena Benchmark Ultra autostainer. The best results were obtained using EDTA pH9 in a steamer then placed on the autostainer, or alternately using CC1 at 99 degrees C for 36 minutes. Ventena Ultraview/DAB was used for the detection system.
Data di pubblicazione: 2018-06-19
Rated 5 di 5 di da Clean and Great expression of TCR Delta. I struggled for over a year to find a replacement for TCR Gamma, which was discontinued by Thermo last year. This is a great replacement for it. I can't upload a TIFF image, but the examples that I wanted to upload were from a Spleen and Colon. The retrieval for Leica Bond III was Hier2 for 40 minutes with 15 minutes of antibody incubation (modified F Protocol). Dilution that worked best was 1:200 (using Leica's diluent).
Data di pubblicazione: 2017-12-20
Rated 5 di 5 di da Good for human detection Positive signal obtained at predicted molecular weight in WB for TCR delta.
Data di pubblicazione: 2017-02-07
Rated 3 di 5 di da Faint band at correct MW in HeLa whole cell lysate Faint band at correct MW in HeLa whole cell lysate. -SCBT QC
Data di pubblicazione: 2013-12-12
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