SRA Antibody (E-5): sc-393240

SRA Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects SRA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-SRA antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The steroid receptor RNA activator (SRA) plays a crucial role in mediating the transactivation of steroid hormone receptors, functioning as both an RNA transcript that forms a complex with steroid receptor coactivator-1 and as a stably expressed protein. SRA contains six RNA motifs essential for coactivation capabilities, which are vital for the regulation of gene expression in response to steroid hormones. SRA is ubiquitously expressed across normal tissues, with particularly high levels found in the liver and skeletal muscle, while exhibiting lower expression in the brain. SRA is overexpressed in breast tumors compared to normal tissue, suggesting a potential role in cancer biology. SRA overexpression enhances estrogen receptor alpha transcriptional activity, and when cells are transfected with antisense oligodeoxynucleotides targeting SRA, a dose-dependent reduction in estrogen receptor alpha expression occurs. The SMRT/HDAC1 associated repressor protein (SHARP) interacts with SRA, inhibiting SRA′s ability to potentiate steroid receptor transcription, highlighting complex regulatory mechanisms in cellular signaling pathways.

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SRA Antibody (E-5) References:

1. A steroid receptor coactivator, SRA, functions as an RNA and is present in an SRC-1 complex.  |  Lanz, RB., et al. 1999. Cell. 97: 17-27. PMID: 10199399
2. Altered expression of estrogen receptor coregulators during human breast tumorigenesis.  |  Murphy, LC., et al. 2000. Cancer Res. 60: 6266-71. PMID: 11103781
3. A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor alpha coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRA.  |  Watanabe, M., et al. 2001. EMBO J. 20: 1341-52. PMID: 11250900
4. Sharp, an inducible cofactor that integrates nuclear receptor repression and activation.  |  Shi, Y., et al. 2001. Genes Dev. 15: 1140-51. PMID: 11331609
5. Reduction of coactivator expression by antisense oligodeoxynucleotides inhibits ERalpha transcriptional activity and MCF-7 proliferation.  |  Cavarretta, IT., et al. 2002. Mol Endocrinol. 16: 253-70. PMID: 11818499
6. Distinct RNA motifs are important for coactivation of steroid hormone receptors by steroid receptor RNA activator (SRA).  |  Lanz, RB., et al. 2002. Proc Natl Acad Sci U S A. 99: 16081-6. PMID: 12444263
7. Identification of new human coding steroid receptor RNA activator isoforms.  |  Emberley, E., et al. 2003. Biochem Biophys Res Commun. 301: 509-15. PMID: 12565891
8. Ligand-independent coactivation of ERalpha AF-1 by steroid receptor RNA activator (SRA) via MAPK activation.  |  Deblois, G. and Giguère, V. 2003. J Steroid Biochem Mol Biol. 85: 123-31. PMID: 12943696
9. Expression and function of human steroid receptor RNA activator in prostate cancer cells: role of endogenous hSRA protein in androgen receptor-mediated transcription.  |  Kurisu, T., et al. 2006. Prostate Cancer Prostatic Dis. 9: 173-8. PMID: 16607388
10. Alternative splicing of the first intron of the steroid receptor RNA activator (SRA) participates in the generation of coding and noncoding RNA isoforms in breast cancer cell lines.  |  Hube, F., et al. 2006. DNA Cell Biol. 25: 418-28. PMID: 16848684
11. Pus3p- and Pus1p-dependent pseudouridylation of steroid receptor RNA activator controls a functional switch that regulates nuclear receptor signaling.  |  Zhao, X., et al. 2007. Mol Endocrinol. 21: 686-99. PMID: 17170069
12. Influence of ovarian endometrioma on expression of steroid receptor RNA activator, estrogen receptors, vascular endothelial growth factor, and thrombospondin 1 in the surrounding ovarian tissues.  |  Lin, K., et al. 2014. Reprod Sci. 21: 183-9. PMID: 23749764

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SRA Antibody (E-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-SRA Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SRA Antibody (E-5) Specifications

Alternate Product Name: SRA

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-220

Epitope Target: SRA

Epitope Species: mouse

Product Reactivity/Detection: SRA

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for SRA Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin