SPATA2 Antibody (B-7): sc-515283

SPATA2 Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects SPATA2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPATA2 Antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPATA2, or spermatogenesis associated protein 2, is a 520 amino acid nuclear protein predominantly expressed in Sertoli cells of the testis, where SPATA2 plays a crucial role in regulating spermatogenesis. Notably, SPATA2 is not found in spermatogenic cells, indicating SPATA2′s specific function in supporting the environment necessary for sperm development. SPATA2′s expression is significantly upregulated in response to follicle-stimulating hormone (FSH), which is essential for Sertoli cell function, suggesting SPATA2 is an FSH-responsive protein that may influence Sertoli cell activity and, consequently, spermatogenesis. Additionally, SPATA2 shares approximately 85% sequence identity with its rat homolog, highlighting SPATA2′s evolutionary conservation across mammals and underscoring SPATA2′s potential importance in reproductive biology.

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SPATA2 Antibody (B-7) References:

1. cDNA cloning and characterization of PD1: a novel human testicular protein with different expressions in various testiculopathies.  |  Graziotto, R., et al. 1999. Exp Cell Res. 248: 620-6. PMID: 10222154
2. A novel gene (PD1) with a potential role on rat spermatogenesis.  |  Onisto, M., et al. 2000. J Endocrinol Invest. 23: 605-8. PMID: 11079456
3. Evidence for FSH-dependent upregulation of SPATA2 (spermatogenesis-associated protein 2).  |  Onisto, M., et al. 2001. Biochem Biophys Res Commun. 283: 86-92. PMID: 11322771
4. Cloning and characterization of the promoter region of human spata2 (spermatogenesis-associated protein 2) gene.  |  Liliana Slongo, M., et al. 2003. Biochim Biophys Acta. 1625: 192-6. PMID: 12531478
5. Zebrafish spata2 is expressed at early developmental stages.  |  Moro, E., et al. 2007. Int J Dev Biol. 51: 241-6. PMID: 17486545
6. Identification of ZNF313/RNF114 as a novel psoriasis susceptibility gene.  |  Capon, F., et al. 2008. Hum Mol Genet. 17: 1938-45. PMID: 18364390
7. SPATA2 promotes CYLD activity and regulates TNF-induced NF-κB signaling and cell death.  |  Schlicher, L., et al. 2016. EMBO Rep. 17: 1485-1497. PMID: 27458237
8. SPATA2: more than a missing link.  |  Schlicher, L., et al. 2017. Cell Death Differ. 24: 1142-1147. PMID: 28282038
9. The SPATA2/CYLD pathway contributes to doxorubicin-induced cardiomyocyte ferroptosis via enhancing ferritinophagy.  |  Zhou, YJ., et al. 2022. Chem Biol Interact. 368: 110205. PMID: 36195186
10. The role of SPATA2 in TNF signaling, cancer, and spermatogenesis.  |  Masola, V., et al. 2022. Cell Death Dis. 13: 977. PMID: 36402749
11. SPATA2 and CYLD inhibit T cell infiltration into colorectal cancer via regulation of IFN-γ/STAT1 axis.  |  Tan, TG., et al. 2022. Front Oncol. 12: 1016307. PMID: 36531014
12. Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.  |  Nagase, T., et al. 1998. DNA Res. 5: 277-86. PMID: 9872452

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SPATA2 Antibody (B-7) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-SPATA2 Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SPATA2 Antibody (B-7) Specifications

Alternate Product Name: SPATA2

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 417-520

Epitope Target: SPATA2

Epitope Species: human

Product Reactivity/Detection: SPATA2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for SPATA2 Antibody (B-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin