Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SDF-4 CRISPR/Cas9 KO Plasmid (m): sc-422857
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SDF-4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SDF-4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SDF-4 Antibody (E-12): sc-393930
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SDF-4 CRISPR/Cas9 KO Plasmid (m) | sc-422857 | 20 µg | $397.00 |
Overview
Sdf4 encodes stromal cell–derived factor 4 (SDF-4), an endoplasmic reticulum–resident protein implicated in calcium homeostasis and secretory pathway function. SDF-4 is associated with ER quality control and protein trafficking processes that influence vesicular transport and cellular stress responses. Through these roles, Sdf4 can affect signaling programs linked to secretion-dependent cell communication and tissue homeostasis. Dysregulation of ER calcium handling and proteostasis is relevant to mechanisms underlying metabolic dysfunction, neurodegeneration, and inflammatory phenotypes, making Sdf4 a useful node for pathway-focused studies.
SDF-4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sdf4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sdf4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sdf4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SDF-4 protein expression.
This CRISPR knockout system enables efficient generation of Sdf4-deficient cell models for investigation of SDF-4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sdf4 exon(s) critical for SDF-4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sdf4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SDF-4 CRISPR/Cas9 KO Plasmid (m) and SDF-4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sdf4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SDF-4 HDR Plasmid (m) and SDF-4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sdf4 homology arms to support homology-directed repair at defined Sdf4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.