S-100 β chain CRISPR/Cas9 KO Plasmid (h): sc-400707

Overview

S100B encodes the S-100 beta chain, a calcium-binding protein of the S100 family that modulates intracellular Ca2+ signaling and extracellular receptor-mediated responses in a cell context–dependent manner. It participates in regulation of cytoskeletal dynamics, cell cycle progression, oxidative stress responses, and inflammatory signaling, including interactions that influence MAPK/ERK and NF-κB–linked processes. S-100β is highly studied in astrocyte biology and neuroinflammation, and altered expression has been associated with neurological injury and neurodegenerative conditions as well as tumor microenvironment signaling in certain cancers. These functions make S100B a useful node for dissecting glial activation states, stress signaling, and cell–cell communication pathways.

S-100 β chain CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the S100B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the S100B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the S100B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish S-100 β chain protein expression.

This CRISPR knockout system enables efficient generation of S100B-deficient cell models for investigation of S-100 β chain signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting S100B exon(s) critical for S-100 β chain function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple S100B genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by S-100 β chain CRISPR/Cas9 KO Plasmid (h) and S-100 β chain CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the S100B locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by S-100 β chain HDR Plasmid (h) and S-100 β chain HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by S100B homology arms to support homology-directed repair at defined S100B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.