Anticorpo PTP1B (H-9) raccomandato per il rilevamento di PTP1B di origine human in WB, IP, IF, IHC(P) e ELISA

Anticorpo PTP1B (H-9): sc-133258

Anticorpo PTP1B (H-9) valutazione 5.0 di 5 di 5.
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Schede tecniche

    • PTP1B Antibody H-9 è un monoclonale di topo IgG1 fornito in 200 µg/ml
    • raised against amino acids 301-435 mapping at the C-terminus of PTP1B (protein tyrosine phosphatase) of human origin
    • raccomandato per il rilevamento di PTP1B di origine human in WB, IP, IF, IHC(P) e ELISA
    • Vedere PTP1B (D-4): sc-133259 for PTP1B per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PTP1B Antibody (H-9) for WB and IHC(P) applications. These reagents are now offered in bundles with PTP1B Antibody (H-9) (see ordering information below).
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I am using PTP1B (H-9): sc-133258 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Richiesta da: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
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Rated 5 di 5 di da Nice antibody for western blot bands We used this antibody for western blot in order to detect PTP1B expression. We got positive bands shown up at the expected molecular weight in Jurkat cells.
Data di pubblicazione: 2017-01-27
Rated 5 di 5 di da Strong band seen in WB in HL Strong band seen in WB in HL-60 whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-05-04
Rated 5 di 5 di da Good antibody for WB in JAR whole cell lysate Good antibody for WB in JAR whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-03-29
Rated 5 di 5 di da Great immunofluorescence cytoplasmic staining Great immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Data di pubblicazione: 2015-02-20
Rated 5 di 5 di da Positive band in WB in human PTP1B transfected Positive band in WB in human PTP1B transfected 293 whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-01-15
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