PPP1R6 CRISPR/Cas9 KO Plasmid (h): sc-406901
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PPP1R6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PPP1R6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPP1R6 CRISPR/Cas9 KO Plasmid (h) | sc-406901 | 20 µg | $397.00 |
Overview
PPP1R3D encodes the regulatory subunit PPP1R6, a glycogen-targeting adaptor that directs protein phosphatase 1 (PP1) toward substrates involved in carbohydrate storage and energy homeostasis. By modulating PP1 localization and catalytic access, PPP1R6 can influence the phosphorylation state of glycogen-metabolic enzymes and thereby shape glycogen synthesis and mobilization in human cells. This regulatory node intersects with nutrient-sensing and kinase/phosphatase signaling networks that coordinate metabolic flux, cellular growth conditions, and stress responses. Dysregulated glycogen handling and phosphatase signaling are recurrent features of metabolic and proliferative pathologies, making PPP1R6 a useful entry point for mechanistic studies of phosphoregulation in disease-relevant contexts.
PPP1R6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PPP1R3D gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PPP1R3D together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PPP1R3D open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PPP1R6 protein expression.
This CRISPR knockout system enables efficient generation of PPP1R3D-deficient cell models for investigation of PPP1R6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PPP1R3D exon(s) critical for PPP1R6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PPP1R3D genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PPP1R6 CRISPR/Cas9 KO Plasmid (h) and PPP1R6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PPP1R3D locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PPP1R6 HDR Plasmid (h) and PPP1R6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PPP1R3D homology arms to support homology-directed repair at defined PPP1R3D target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.