Anticorpo Polycystin-1 (7E12) raccomandato per il rilevamento di Polycystin-1 di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo Polycystin-1 (7E12): sc-130554

Anticorpo Polycystin-1 (7E12) valutazione 4.8 di 5 di 6.
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Schede tecniche

    • Anti-Polycystin-1 Antibody 7E12 è un monoclonale di topo IgG1 (kappa light chain) Polycystin-1 antibody fornito in 200 µg/ml
    • raised against amino acids 24-180 corresponding to the N-terminal leucine rich domain of Polycystin-1 of human origin
    • Polycystin-1 Antibody (7E12) é raccomandato per il rilevamento di Polycystin-1 di origine mouse, rat e human in WB, IP, IF e ELISA
    • Polycystin-1 Antibody (7E12) è disponibile coniugato ad agarosio per IP; e con phycoerythrin o fluorosceina per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Vedi m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), le nostre alternative ricombinanti ai reagenti secondari convenzionali anti-mouse IgG altamente raccomandate.
    • Contattaci per ricevere un FREE 10 µg sample di Polycystin-1 (7E12): sc-130554.

What's the gel percentage used for this polycystin-1 antibody in WB? Also, is there any suitable reference protein or markers?

Richiesta da: yybio
Thank you for your question. We used a 4-6% gel for this very large protein. These will be very fragile, so handle carefully. Please contact our Technical Service Department for suggestions of suitable high molecular weight proteins that could be used as a reference or molecular weight marker.
Risposta da: Technical Support
Data di pubblicazione: 2019-08-17

Can you provide with a FACS protocol for this antibody (polycystin-1, 7E12) on cell lines?

Richiesta da: Curious2
Thank you for your question. We do not have a specific FACS protocol for use with this antibody. Please check our general protocols online, or contact our Technical Support for more information.
Risposta da: Technical Support
Data di pubblicazione: 2019-04-13

I recently ordered this mAb conjugated to PE. I'm attempting to use this mAb to quantitate a cell surface antigen - to do that I need to know the ratio of PE molecules per mAb. I.E., do you know if there is one PE bound to each mAb, or two, etc?

Richiesta da: Jeff R
Thank you for your question. For PE conjugated antibodies, the ratio is 1:1, so there is 1 PE per antibody molecule.
Risposta da: Tech Service
Data di pubblicazione: 2019-03-25

Which part does this antibody (polycystin-1, 7E12) recognize?

Richiesta da: yybio
Thank you for your question. This monoclonal antibody was raised against amino acids 24-180 corresponding to the N-terminal leucine rich domain of Polycystin-1 of human origin. We will update our product description for future reference.
Risposta da: Technical Support
Data di pubblicazione: 2018-12-10

Is it possible to use the AC conjugated Polycystin-1 Antibody (7E12): sc-130554 AC in both Western Blot and IP?

Richiesta da: jenni
Thank you for your inquiry. We do not recommend the use of the AC conjugated form in Western blot. The AC conjugated form of the antibody is specifically used for IP. The unconjugated form of the antibody is the best choice when working in multiple applications.
Risposta da: Technical Support 3
Data di pubblicazione: 2017-01-09
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Rated 5 di 5 di da Good for your Western blot! An excellent detection reagent for your WB. If you need to visualize polycystin-1 in human samples, this antibody can do it. Provides a nice clear band and has been cited over ten times.
Data di pubblicazione: 2017-01-16
Rated 4 di 5 di da Strong western Very nice western blot data; strong signal with low background
Data di pubblicazione: 2017-01-06
Rated 5 di 5 di da Used this antibody in WB and got good results Used this antibody in WB and got good results.
Data di pubblicazione: 2015-08-17
Rated 5 di 5 di da Successful application of this antibody in Successful application of this antibody in WB using human PDL fibroblasts -SCBT Publication Review
Data di pubblicazione: 2015-04-19
Rated 5 di 5 di da WB publishable data with mIMCD cells WB publishable data with mIMCD cells - SCBT Publication Review
Data di pubblicazione: 2015-03-30
Rated 5 di 5 di da This antibody was used in a publication for This antibody was used in a publication for WB on human HEK293 cells - SCBT Publication Review
Data di pubblicazione: 2015-03-18
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