Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
paxillin Double Nickase Plasmid (m): sc-422546-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- paxillin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- paxillin Double Nickase Plasmid (m) and paxillin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Pxn. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: paxillin Antibody (B-2): sc-365379
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
paxillin Double Nickase Plasmid (m) | sc-422546-NIC | 20 µg | $410.00 | |||
paxillin Double Nickase Plasmid (m2) | sc-422546-NIC-2 | 20 µg | $410.00 |
Mouse Pxn encodes paxillin, a multidomain focal adhesion adaptor that coordinates integrin-dependent signaling and actin cytoskeletal remodeling during cell adhesion, spreading, and migration. Paxillin serves as a scaffold for kinases and GTPase regulators, integrating cues from FAK/SRC and downstream RHO-family pathways to modulate focal adhesion turnover and mechanotransduction. Through these functions it influences extracellular matrix sensing, lamellipodia dynamics, and cell polarity. Dysregulated paxillin signaling and focal adhesion remodeling are frequently implicated in altered motility and invasive phenotypes in disease-relevant models, making Pxn a useful node for studying adhesion-associated signaling networks.
paxillin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Pxn locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Pxn. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Pxn function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Pxn-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.