PAR-2 Antibody (SAM11): sc-13504

PAR-2 Antibody (SAM11) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect Proteinase-Activated Receptor 2 (PAR-2), also known as F2RL1 or PAR2, across mouse, rat, and human species using applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). PAR-2 monoclonal antibody (SAM11) targets an epitope mapped within amino acids 37-50 of the human PAR-2 protein, enabling precise identification and analysis of PAR-2 in various experimental settings. PAR-2 functions as a G protein-coupled receptor that plays a pivotal role in mediating inflammatory responses and pain perception by interacting with extracellular proteases like trypsin, which cleave and activate the receptor. Located on the cell surface, PAR-2 facilitates critical signal transduction pathways that regulate cellular responses to environmental stimuli, making its cellular localization essential for its role in physiological and pathological processes. Structurally, PAR-2 is characterized by its seven transmembrane domains, a feature that is crucial for its interaction with G proteins and subsequent intracellular signaling cascades. Understanding the structure of PAR-2 is vital for elucidating its mechanism of action and for the development of targeted therapeutics. PAR-2 monoclonal antibody (SAM11) is available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing versatile options for diverse research applications. PAR-2 monoclonal antibody (SAM11) is an invaluable tool for researchers investigating the molecular mechanisms of PAR-2-mediated cellular functions and its implications in diseases such as inflammation, making a critical component in advancing biomedical research.

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PAR-2 Antibody (SAM11) References:

1. Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin.  |  Kahn, ML., et al. 1999. J Clin Invest. 103: 879-87. PMID: 10079109
2. PAR-1 and PAR-2 Expression Is Enhanced in Inflamed Odontoblast Cells.  |  Alvarez, MMP., et al. 2017. J Dent Res. 96: 1518-1525. PMID: 28759300
3. Mice expressing fluorescent PAR2 reveal that endocytosis mediates colonic inflammation and pain.  |  Latorre, R., et al. 2022. Proc Natl Acad Sci U S A. 119: PMID: 35110404
4. Matriptase drives dissemination of ovarian cancer spheroids by a PAR-2/PI3K/Akt/MMP9 signaling axis.  |  Pawar, NR., et al. 2023. J Cell Biol. 222: PMID: 37737895
5. Involvement of PAR-2 in the Induction of Cell-Specific Matrix Metalloproteinase-2 by Activated Protein C in Cutaneous Wound Healing.  |  Julovi, SM., et al. 2023. Int J Mol Sci. 25: PMID: 38203540
6. Evidence for the presence of a protease-activated receptor distinct from the thrombin receptor in human keratinocytes.  |  Santulli, RJ., et al. 1995. Proc Natl Acad Sci U S A. 92: 9151-5. PMID: 7568091
7. Agonist recognition by proteinase-activated receptor 2 and thrombin receptor. Importance of extracellular loop interactions for receptor function.  |  Lerner, DJ., et al. 1996. J Biol Chem. 271: 13943-7. PMID: 8662993
8. The proteinase-activated receptor 2 is induced by inflammatory mediators in human endothelial cells. Comparison with the thrombin receptor.  |  Nystedt, S., et al. 1996. J Biol Chem. 271: 14910-5. PMID: 8663011
9. The human proteinase-activated receptor-3 (PAR-3) gene. Identification within a Par gene cluster and characterization in vascular endothelial cells and platelets.  |  Schmidt, VA., et al. 1998. J Biol Chem. 273: 15061-8. PMID: 9614115
10. Cloning and characterization of human protease-activated receptor 4.  |  Xu, WF., et al. 1998. Proc Natl Acad Sci U S A. 95: 6642-6. PMID: 9618465
11. Thrombin.  |  Goldsack, NR., et al. 1998. Int J Biochem Cell Biol. 30: 641-6. PMID: 9695019
12. Analysis of a Ca2+-activated K+ channel that mediates hyperpolarization via the thrombin receptor pathway.  |  Sullivan, R., et al. 1998. Am J Physiol. 275: C1342-8. PMID: 9814983

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PAR-2 Antibody (SAM11) has 263 citations in a variety of scientific publications.

Primary antibodies like Anti-PAR-2 Antibody (SAM11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PAR-2 Antibody (SAM11) Specifications

Alternate Product Name: PAR2

Product Clone Name: SAM11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 37-50

Epitope Target: PAR-2

Epitope Species: human

Product Reactivity/Detection: PAR-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 263 citations in various scientific publications

Available Conjugates/Forms for PAR-2 Antibody (SAM11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin