p-Myc Antibody (E-3): sc-377552

p-c-Myc Antibody (E-3) is a mouse monoclonal IgG2b kappa light chain antibody raised against human c-Myc dually phosphorylated at Thr58 and Ser62, a crucial transcription factor involved in regulating cell proliferation, differentiation, and neoplastic transformation. p-c-Myc mouse monoclonal antibody (E-3) specifically recognizes c-Myc both phosporylated Thr58 and Ser62, and shows broad species cross-reactivity in Western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), and enzyme-linked immunosorbent assay applications. Anti-p-c-Myc monoclonal antibody (E-3) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. The phosphorylation of these sites represents a sophisticated regulatory mechanism: Ser62 phosphorylation, mediated by ERK kinases in response to growth signals, initially stabilizes c-Myc and enhances its transcriptional activity. This modification then serves as a priming event for subsequent Thr58 phosphorylation by GSK-3, which triggers protein degradation through the ubiquitin-proteasome pathway. This phosphorylation cascade ensures precise temporal control of c-Myc activity, with disruption of this regulatory mechanism, particularly through mutations affecting Thr58 phosphorylation, frequently observed in cancers such as Burkitt′s lymphoma. The c-Myc protein contains three distinct motifs in its COOH terminus: the leucine zipper, the helix-loop-helix, and a basic region, which together enable sequence-specific DNA binding. This DNA-binding activity is enhanced through complex formation with Max protein, a basic region helix-loop-helix leucine zipper protein that also interacts with other bHLH-Zip proteins such as Mad1 and Mxi1. The dynamic interplay between these proteins regulates gene expression, as Mad1-Max dimers can repress transcription by recruiting co-repressors like mSin3, influencing cellular outcomes in both normal and pathological states. Amplification of the c-Myc gene occurs frequently in various human tumors, including lung, breast, and colon cancers, highlighting c-Myc′s critical role in oncogenesis. p-c-Myc (E-3) antibody serves as an indispensable tool for exploring these intricate regulatory mechanisms of c-Myc in transcriptional control, oncogenesis, and cellular homeostasis.

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p-Myc Antibody (E-3) References:

1. Phosphorylated c-MYC expression in Alzheimer disease, Pick's disease, progressive supranuclear palsy and corticobasal degeneration.  |  Ferrer, I., et al. 2001. Neuropathol Appl Neurobiol. 27: 343-51. PMID: 11679086
2. Transcriptional activation by the human c-Myc oncoprotein in yeast requires interaction with Max.  |  Amati, B., et al. 1992. Nature. 359: 423-6. PMID: 1406955
3. Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants.  |  Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-92. PMID: 1644290
4. Nucleolus disassembly in mitosis and apoptosis: dynamic redistribution of phosphorylated-c-Myc, fibrillarin and Ki-67.  |  Soldani, C., et al. 2006. Eur J Histochem. 50: 273-80. PMID: 17213035
5. Effect of paclitaxel on intracellular localization of c-Myc and P-c-Myc in prostate carcinoma cell lines.  |  Supino, R., et al. 2007. Ann N Y Acad Sci. 1095: 175-81. PMID: 17404030
6. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc.  |  Blackwood, EM. and Eisenman, RN. 1991. Science. 251: 1211-7. PMID: 2006410
7. Enhanced expression of the N-myc gene in Wilms' tumors.  |  Nisen, PD., et al. 1986. Cancer Res. 46: 6217-22. PMID: 2430692
8. L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer.  |  Nau, MM., et al. Nature. 318: 69-73. PMID: 2997622
9. Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuroendocrine cells from a human colon carcinoma.  |  Alitalo, K., et al. 1983. Proc Natl Acad Sci U S A. 80: 1707-11. PMID: 6300869
10. Mad-Max transcriptional repression is mediated by ternary complex formation with mammalian homologs of yeast repressor Sin3.  |  Ayer, DE., et al. 1995. Cell. 80: 767-76. PMID: 7889570

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Myc Antibody (E-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Myc Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Myc Antibody (E-3) Specifications

Alternate Product Name: Myc

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Thr 58/Ser 62

Epitope Target: c-Myc

Epitope Species: human

Product Reactivity/Detection: Thr 58 and Ser 62 dually phosphorylated c-Myc

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for p-Myc Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Myc antibody is Myc Antibody (9E10): sc-40.