Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
OMP Double Nickase Plasmid (m): sc-422061-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OMP Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OMP Double Nickase Plasmid (m) and OMP Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Omp. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OMP Antibody (B-6): sc-365818
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OMP Double Nickase Plasmid (m) | sc-422061-NIC | 20 µg | $410.00 |
Mouse Omp encodes olfactory marker protein (OMP), a cytosolic protein highly enriched in mature olfactory sensory neurons where it supports efficient odor-evoked signal transduction. OMP modulates neuronal excitability and response kinetics in part by shaping cyclic nucleotide–dependent signaling and calcium dynamics downstream of odorant receptor activation in the olfactory cilia. Through its role in sensory neuron maturation and synaptic function within olfactory circuits, altered Omp expression or function is used to study mechanisms underlying hyposmia/anosmia and broader neurodevelopmental or neurodegenerative contexts where sensory processing is affected. Omp is therefore a widely used molecular marker and functional node for dissecting olfactory pathway biology and neuron state transitions.
OMP Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Omp locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Omp. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Omp function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Omp-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.